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Utilization of an Alternative Carbon Source for Efficient Production of Human α1-Antitrypsin by Genetically Engineered Rice Cell Culture

Authors

  • Masaaki Terashima,

    Corresponding author
    1. Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1–1, Gakuen-cho, Sakai, 599–8531, Osaka Japan
    • Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1–1, Gakuen-cho, Sakai, 599–8531, Osaka Japan. Ph: +81–722–54–9301
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  • Yoko Ejiri,

    1. Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1–1, Gakuen-cho, Sakai, 599–8531, Osaka Japan
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  • Naohiro Hashikawa,

    1. Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1–1, Gakuen-cho, Sakai, 599–8531, Osaka Japan
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  • Hiroyuki Yoshida

    1. Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1–1, Gakuen-cho, Sakai, 599–8531, Osaka Japan
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Abstract

Human α1-antitrypsin was produced by genetically engineered rice cells using promoter and signal peptide of a rice α-amylase isozyme. Batch and continuous cultures were employed to investigate the effects of alternative carbon sources on the α1-antitrypsin production. While this expression system is inducible by sugar depletion, we have found that the productivity of α1-antitrypsin increased 2.4- to 3.4-fold, compared with the control medium without carbon source, in medium containing an alternative carbon source, such as pyruvic acid and glyoxylic acid. The accumulated α1-antitrypsin in the medium containing pyruvic acid reached 18.2−24.2 mg/g-dry cell in 50−70 h by batch culture.

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