Get access

Water Content in an Engineered Dermal Replacement during Permeation of Me2SO Solutions Using Rapid MR Imaging

Authors

  • Nicolas P. Bidault,

    1. Departments of Biomedical Engineering, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 292, Minneapolis, Minnesota 55455
    2. Departments of Radiology, CIA-MR, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 292, Minneapolis, Minnesota 55455
    3. Proctor & Gamble Rome Technical Center, via della Maglianella 651, 00 166 Rome, Italy
    Search for more papers by this author
  • Bruce E. Hammer,

    1. Departments of Biomedical Engineering, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 292, Minneapolis, Minnesota 55455
    2. Departments of Radiology, CIA-MR, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 292, Minneapolis, Minnesota 55455
    Search for more papers by this author
  • Allison Hubel

    Corresponding author
    1. Departments of Biomedical Engineering, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 292, Minneapolis, Minnesota 55455
    • Departments of Biomedical Engineering, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 292, Minneapolis, Minnesota 55455. Ph: 612/626–2366
    Search for more papers by this author

Abstract

The successful cryopreservation of cell and tissues typically requires the use of specialized solutions containing cryoprotective agents. At room temperature, the introduction of a cryopreservation solution can result in cell damage/death resulting from osmotic stresses and/or biochemical toxicity of the solution. For tissues, the permeation and equilibration of a cryoprotective solution throughout the tissue is important in enhancing the uniformity and consistency of the postthaw viability of the tissue. Magnetic resonance (MR) is a common nondestructive technique that can be used to quantitate the temporal and spatial composition of water and cryoprotective agents in a three-dimensional system. We have applied a recently developed rapid NMR imaging technique to quantify the transport of water in an artificial dermal replacement upon permeation of dimethyl sulfoxide (Me2SO) solutions. Results indicate that the rate of water transport is slower in the presence of Me2SO molecules. Furthermore, the transport is concentration-dependent, suggesting that Me2SO tends to retain bound water molecules in the tissue. Moreover, water transport decreases with decreasing temperature, and the presence of cells tends to increase water transport.

Get access to the full text of this article

Ancillary