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Monitoring a Complex Medium Fermentation with Sample-Sample Two-Dimensional FT-NIR Correlation Spectroscopy

Authors

  • Ana P. Ferreira,

    1. Centre for Biological and Chemical Engineering, IST, Technical University of Lisbon, Av. Rovisco Pais, P-1049–001 Lisbon, Portugal
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  • José C. Menezes

    Corresponding author
    1. Centre for Biological and Chemical Engineering, IST, Technical University of Lisbon, Av. Rovisco Pais, P-1049–001 Lisbon, Portugal
    • Centre for Biological and Chemical Engineering, IST, Technical University of Lisbon, Av. Rovisco Pais, P-1049–001 Lisbon, Portugal. Tel: (+351) 21 841 9838. Fax: (+351) 21 841 9197
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Abstract

The possibility of monitoring state transitions during the time course of a fermentation through the analysis of the on-line collected near-infrared (NIR) spectra with sample-sample two-dimensional correlation spectroscopy (SS-2DCoS) was investigated. SS-2DCoS has proved to be useful for extracting process information directly from the spectra collected on-line. The complexity of the system studied prevented the extraction of concentration profiles, but nevertheless, the application of SS-2DCoS enables the identification of fermentation state transitions due to metabolic and morphological changes of the microorganism.

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