Development of a Method Using Image Analysis for the Measurement of Cellulose-Binding Domains Adsorbed onto Cellulose Fibers

Authors

  • Ricardo Pinto,

    1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal
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  • António L. Amaral,

    1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal
    2. Departamento de Tecnologia Química, ESTIG, IPB, Apartado 1038, 5301–854 Bragança, Portugal
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  • Joana Carvalho,

    1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal
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  • Eugénio C. Ferreira,

    1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal
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  • Manuel Mota,

    1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal
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  • Miguel Gama

    Corresponding author
    1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal
    • IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710–057, Braga, Portugal. Fax: +351253678986
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Abstract

The surface concentration of CBD-FITC conjugates, adsorbed on cellulose fibers, was determined by image analysis. The program consists of two scripts, the first dedicated to the elaboration of the calibration curve. The emission of fluorescent light, detected by image analysis, is correlated with the concentration of CBD solutions. This calibration is then used (second script) to determine the concentration of CBDs adsorbed on cellulosic fibers. This method allows the direct estimation of the surface concentration of adsorbed CBDs, which usually is not accurately calculated from depletion studies, since the surface area is hardly known. By observing different spots in the surface of the fibers, site-specific information is obtained. It was verified that the physically heterogeneous fibers exhibit different amounts of adsorbed CBDs.

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