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Enzymic Thin Film Coatings for Bioactive Materials

  1. Xiaodong Tong1,†,
  2. Archana Trivedi1,†,
  3. Hongfei Jia3,
  4. Minjuan Zhang3,
  5. Ping Wang1,2,*

Article first published online: 5 SEP 2008

DOI: 10.1021/bp0704135

Issue

Biotechnology Progress

Biotechnology Progress

Volume 24, Issue 3, pages 714–719, May/June 2008

Additional Information

How to Cite

Tong, X., Trivedi, A., Jia, H., Zhang, M. and Wang, P. (2008), Enzymic Thin Film Coatings for Bioactive Materials. Biotechnol Progress, 24: 714–719. doi: 10.1021/bp0704135

Author Information

  1. 1

    Biotechnology Institute and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108

  2. 2

    Department of Chemical and Biomolecular Engineering, University of Akron, Akron, Ohio 44325

  3. 3

    Materials Research Department, Toyota Motor Engineering and Manufacturing North America, Inc., Ann Arbor, Michigan 48105

  1. †

    Contributed equally to this work

*Biotechnology Institute and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108. Tel: +1–612–6244792

  1. †

    Contributed equally to this work

Publication History

  1. Issue published online: 5 SEP 2008
  2. Article first published online: 5 SEP 2008
  3. Manuscript Accepted: 24 MAR 2008
  4. Manuscript Received: 30 OCT 2007

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Abstract

Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 μg/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.

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