Glycerol Production in a Triose Phosphate Isomerase Deficient Mutant of Saccharomyces cerevisiae

Authors

  • Concetta Compagno,

    Corresponding author
    1. Dipartimento di Fisiologia e Biochimica Generali, Sez. Biochimica Comparata, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy
    • Dipartimento di Fisiologia e Biochimica Generali, Sez. Biochimica Comparata, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy
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  • Francesco Boschi,

    1. Dipartimento di Fisiologia e Biochimica Generali, Sez. Biochimica Comparata, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy
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  • Bianca Maria Ranzi

    1. Dipartimento di Fisiologia e Biochimica Generali, Sez. Biochimica Comparata, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy
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Abstract

Interesting challenges from metabolically engineered Saccharomyces cerevisiae cells arise from the opportunity to obtain yeast strains useful for the production of chemicals. In this paper, we show that engineered yeast cells deficient in the triose phosphate isomerase activity are able to produce glycerol without the use of steering agents. High yields of conversion of glucose into glycerol (80−90% of the theoretical yield) and productivity (1.5 g L−1 h−1) have been obtained by a bioconversion process carried out in a poor and clean medium. We obtained indications that the growth phase at which the biomass was collected affect the process. The best results were obtained using cells collected at the end of exponential phase of growth. In perspective, the strategies and the information about the physiology of the cells described here could be useful for the developing of new biotechnological processes for glycerol production, outflanking the problems related to the use of high level of steering agents.

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