The extension of the shock layer theory to systems having a slow mass transfer kinetics and a concentration-dependent rate coefficient is discussed. Experiments were carried out with bovine serum albumin on two anion exchangers, TSK-GEL-DEAE-5PW and Resource-Q. The adsorption isotherm data, determined by single-step frontal analysis, could be fitted to simplified bi-Langmuir equations with very small residuals. A lumped kinetic model (solid film linear driving force model, with rate coefficient kf) was used to account for the mass transfer kinetics. The profile of each breakthrough curve (BC) was fitted to the curve calculated with this transport model and the rate coefficient kf obtained by identification. A linear dependence of kf on the average concentration of the step of the BC was found. The shock layer thicknesses (SLT) calculated for different relative concentrations agreed very well with the experimental results. This justifies the use of the SLT for the direct determination of rate coefficients.