A multiproxy integrated chronological framework, based on oxygen and carbon stable isotope stratigraphy, biostratigraphy (foraminifera and nannoplankton bioevents and foraminifer assemblage-based climate cyclicity), magnetostratigraphy, sapropel stratigraphy, and 14C AMS radiometric dates, has been achieved for borehole PRAD1-2, collected in 185.5 m water depth in the central Adriatic. This work was carried out within the European Community project Profiles across Mediterranean Sedimentary Systems (PROMESS1). The 71.2 m long borehole spans a time interval between late MIS 11 and MIS 1 (the last 370 ka), showing a chronological resolution of 500 and 250 years per cm during interglacial and glacial intervals, respectively. At present, this record is the most expanded and continuous marine record available for the Adriatic Basin. Several orbital cycles can be recognized in the PRAD1-2 record: the 100 ka glacial-interglacial fluctuations and the 23 ka precession-related cycles, which in turn control the deposition of sapropel layers. An integrated analysis of short-term oscillations within the Last Glaciation interval (MIS 4–MIS 2) allowed the identification of the Adriatic signature of Dansgaard-Oeschger events, showing the potential to achieve a more refined chronostratigraphic framework for the top part of the PRAD1-2 record. Finally, the age model obtained by this study allowed the chronological integration of the main foraminifera bioevents detected in the borehole as well as of the volcanoclastic layers present in the upper part of the record. Despite its proximal location, PRAD1-2 presents a continuous record and shows the potential to be consistently correlated both with deep-sea and continental records in the Mediterranean region and beyond.