We present a species-specific Mg/Ca-calcification temperature calibration for Globorotalia inflata from a suite of 38 core top samples from the South Atlantic (from 8°S to 49°S). G. inflata is a deep-dwelling planktonic foraminifer commonly occurring in subtropical to subpolar conditions, which qualifies it for reconstructions of the permanent thermocline. Apparent calcification depths and calcification temperatures were determined by comparing measured δ18O with equilibrium δ18O of calcite based on water column properties. Based on our core top samples, G. inflata apparent calcification depth is constant throughout the South Atlantic midlatitudes with a depth of 350–400 m within the permanent thermocline. The resulting Mg/Ca-calcification temperature calibration is Mg/Ca = 0.72 ± 0.045/0.042 exp (0.076 ± 0.006 calcification temperature) (r2 = 0.81) and covers the temperature range 3.1°C–16.5°C. We applied our Mg/Ca calibration to gravity core PS2495-3 from the Mid-Atlantic Ridge at ∼41°S to test its validity by reconstructing a low-resolution record covering the last two glacial-interglacial cycles. Our paleotemperature record reveals large changes in temperature for Terminations I and II, when permanent thermocline temperature increased by as much as 8°C. The G. inflata paleotemperature record suggests that oceanic fronts repeatedly migrated over the location of core PS2495-3 during the last 160 kyr. This study shows the potential of G. inflata Mg/Ca to reconstruct paleotemperatures in the permanent thermocline.
Globorotalia inflata is one of the most abundant deep-dwelling transitional water species in the South Atlantic [e.g., Bé and Hutson, 1977; Niebler and Gersonde, 1998]. Its occurrence in core top samples amounts to >20% of the total planktonic foraminiferal assemblage between 30 and 50°S, encompassing the Subtropical Front (STF) and Subantarctic Front (SAF) as well as part of the Polar Frontal Zone (PFZ). During its ontogenetic cycle, G. inflata migrates through the upper few hundred meters of the water column [e.g., Lončarić et al., 2006; Wilke et al., 2006; Chiessi et al., 2007; Cléroux et al., 2007], providing great potential of recording past thermocline conditions [Chiessi et al., 2008] as well as the migration of midlatitude oceanic fronts.
 In this study we present a Mg/Ca-calcification temperature calibration for G. inflata that we derive from core top samples from the South Atlantic. Additionally, we compare Mg/Ca of specimens from different size fractions and different states of encrustation to demonstrate the impact of encrustation on Mg/Ca. We test our calibration on a downcore G. inflata Mg/Ca record from gravity core PS2495-3 raised in the Subantarctic Zone (SAZ) of the Mid-Atlantic Ridge to assess the migration of the STF and the SAF during the last two glacial-interglacial cycles. Our data show that Mg/Ca from G. inflata is a reliable recorder of permanent thermocline temperatures even under considerably different upper water column structures, highlighting its applicability in paleoceanographic studies.
2. Materials and Methods
 We used a set of 38 core top samples from the South Atlantic that were retrieved between 8°S and 49°S, 6°E and 60°W, covering water depths between ∼500 and 3800 m (Figure 1a and Table 1) to establish our Mg/Ca-calcification temperature calibration for G. inflata. For the determination of the apparent calcification depth of G. inflata we also included 22 additional core top samples from the western South Atlantic already published by Chiessi et al. .
Table 1. Surface Sample Locations, G. inflata δ18O, Mg/Ca, Apparent Calcification Depths, and Calcification Temperaturesa
From the World Ocean Atlas 2005 [Locarnini et al., 2006] for each surface sample location at its respective apparent calcification depth.
 Samples were taken from the undisturbed uppermost centimeter of multicores. The late Holocene age of all samples was confirmed by the presence of stained benthic foraminifera [Harloff and Mackensen, 1997; Chiessi et al., 2007; this study]. Additionally, isotope stratigraphy for core GeoB2109-3 [Dürkoop, 1998], and AMS 14C ages for cores GeoB2804-2 and GeoB2805-1 (0 years BP [Mollenhauer et al., 2006]) corroborate the late Holocene age of the samples.
 To test the application of our G. inflata Mg/Ca-calcification temperature calibration Mg/Ca analyses were performed on gravity core PS2495-3 (41.27°S, 14.49°W, 3134 m water depth), raised from the eastern slope of the Mid-Atlantic Ridge (Figure 1a). This test case application is meant to test and demonstrate the feasibility of our approach to reliably reconstruct permanent thermocline temperatures. The core is located between the modern STF (38–42°S) and SAF (∼45°S) [Peterson and Stramma, 1991; Tsuchiya et al., 1994], providing the opportunity to test our new Mg/Ca calibration by assessing the temperature effects associated with migrations of these oceanic features. The original age model of core PS2495-3 is based on 10 calibrated AMS 14C dates for the last ∼30 kyr [Gersonde et al., 2003] and on standard oxygen isotope stratigraphy on the benthic foraminifer Cibicidoides spp. [Mackensen et al., 2001]. For this study we further tuned the original age model of PS2495-3 to the global benthic δ18O stack of Lisiecki and Raymo . All data presented here are stored in the Pangaea data bank (www.pangaea.de), including the old and new age models.
2.2. Selection of G. inflata Size Fraction and Morphology
 Although maximum abundances of G. inflata often occur within the thermocline [e.g., Fairbanks et al., 1982], calcification of the tests takes place from the mixed layer to water depths possibly deeper than 500 m [e.g., Wilke et al., 2006]. Accordingly, the tests integrate the hydrographic signals in their proxy data that they acquired over a considerable range of water depths. Moreover, sediment samples often contain a large variety in morphology of individual G. inflata specimens, that is, from small, juvenile G. inflata to heavily encrusted and much larger specimens (Figure 2). This provides a potential source of bias when using proxies like Mg/Ca. Therefore, we selected only nonencrusted specimens of G. inflata with three chambers in the final whorl [Kennett and Srinivasan, 1983] in the size range 315–400 μm for the establishment of both the Mg/Ca-calcification temperature calibration and the downcore record. We define nonencrusted specimens as those specimens which are not covered by a shiny calcite crust when observed under a binocular microscope. However, SEM analyses showed that even these specimens do contain some calcite crust (Figure 2). To determine the bias introduced on downcore temperature reconstructions when specimens of different size and morphology are mixed, we also analyzed three additional size fractions of nonencrusted specimens (<250 μm, 250–315 μm, and >400 μm) as well as heavily encrusted, shiny specimens (315–400 μm) from four core depths (Holocene, MIS2, MIS5, and MIS6).
2.3. Mg/Ca and Stable Oxygen Isotope Analyses
 Between 20 and 50 tests of G. inflata were selected from the different size fractions from each sample and gently crushed. The shell fragments were then cleaned according to the standard cleaning protocol for foraminiferal Mg/Ca analyses [Barker et al., 2003]. The tests underwent ultrasonic cleaning alternated with washes in deionized water (Seralpur) and methanol, before an oxidizing step was applied, which was neutralized with multiple deionized water washes (Seralpur). After transfer into clean vials a weak acid leach (0.001 M QD HNO3) was applied, and samples were dissolved in 0.075 M QD HNO3. Before dilution samples were centrifuged for 10 min (6000 rpm) to exclude any remaining insoluble particles from the analyses. Samples were diluted with Seralpur water before analysis with an ICP-OES (Perkin Elmer Optima 3300RL with autosampler and ultrasonic nebulizer U-5000 AT (Cetac Technologies Inc.)) at the Department of Geosciences, University of Bremen. Instrumental precision of the ICP-OES was monitored by analysis of an in-house standard solution with a Mg/Ca of 2.93 mmol/mol after every five samples (long-term standard deviation of 0.026 mmol/mol or 0.91%). To allow interlaboratory comparison we analyzed an international limestone standard (ECRM752–1) with a reported Mg/Ca of 3.75 mmol/mol [Greaves et al., 2008]. The long-term average of the ECRM752–1 standard, which was routinely analyzed twice before each batch of 50 samples in every session, is 3.78 mmol/mol (1σ = 0.066 mmol/mol). Analytical precision based on three replicate measurements of each sample for G. inflata was 0.23% for Mg/Ca, while reproducibility of the samples (n = 47; separately cleaned and analyzed during different ICP-OES sessions) was ±0.12 mmol/mol (1σ, ∼3.8%).
 Stable oxygen isotope ratios were determined on the same samples analyzed for Mg/Ca. We measured between 5 and 15 specimens of G. inflata, depending on the size fraction. Specimens were picked together and then separated for either Mg/Ca or stable oxygen isotope analysis. Stable oxygen isotope analyses were performed using a Finnigan MAT 251 mass spectrometer with an automated carbonate preparation device at the Department of Geosciences, University of Bremen. The external standard error of the stable oxygen isotope analyses is <0.06‰. Values are reported relative to the Vienna Pee Dee Belemnite (VPDB), calibrated by using the National Bureau of Standards (NBS) 18, 19, and 20 standards.
2.4. Determination of Calcification Temperatures
 We determined calcification temperatures based on measured δ18O of G. inflata. As deep-dwelling foraminifera like G. inflata have a much larger habitat range than shallow-dwelling foraminifera, it is necessary to first calculate calcification temperatures in order to construct a Mg/Ca-calcification temperature calibration. We first calculated the equilibrium δ18O of calcite (δ18Oequ) for the whole water column above each surface sample location. For this we used mean annual temperatures from the World Ocean Atlas 2005 [Locarnini et al., 2006], δ18Osw from the global gridded data set of LeGrande and Schmidt , and the paleotemperature equation from Shackleton . One δ18Oequ depth profile was generated for each surface sample site based on the closest grid point from the Locarnini et al.  and LeGrande and Schmidt  databases. The δ18Oequ profiles have been calculated for all depth levels of the World Ocean Atlas 2005 down to 1500 m. As no species-specific paleotemperature equation is available for G. inflata we chose the equation of Shackleton  since (1) the apparent calcification disequilibrium is relatively small (0 ± 0.3‰) for deep-dwelling foraminiferal species [Fairbanks et al., 1982; Deuser and Ross, 1989; Wilke et al., 2006] and (2) it correctly predicts the slope of the δ18O – temperature relationship over the entire temperature range present in the oceans for the most commonly used species of planktonic foraminifera [Mulitza et al., 2003]. In a second step, we determined the apparent calcification depth by comparing foraminiferal δ18O with the δ18Oequ profiles (Table 1). Finally, we obtained the calcification temperature (Table 1) from the World Ocean Atlas 2005 [Locarnini et al., 2006] for each surface sample location at its respective apparent calcification depth.
3.1. Calcification Temperatures
 The stable oxygen isotope composition of G. inflata from core top samples varies between 0.43 and 3.03‰ (Table 1). Apparent calcification depths are between 95 and 1033 m with a mean value of 387 m (1σ = 179 m), and calcification temperatures vary between 3.1°C and 16.5°C (Figure 1b and Table 1).
 The spread in apparent calcification depths is highest close to the STF (38–42°S) in the area of the Brazil-Malvinas Confluence (BMC; westernmost South Atlantic). At the BMC the Brazil Current meets the Malvinas (Falkland) Current, resulting in a highly energetic area with frequent formation of eddies [Olson et al., 1988; Stramma and England, 1999]. The resulting strong currents and large-scale eddies can carry planktonic foraminifera away from their natural habitat. Such expatriation may play an important role in the Brazil-Malvinas Confluence [Berger, 1970; Bijma et al., 1990; Boltovskoy, 1994]. Expatriated foraminifera often survive and keep calcifying at water depth ranges that are outside their typical habitat depth [Boltovskoy, 1994]. As a result of the expatriation their apparent calcification depth and calcification temperature will differ from that observed under typical conditions. Nevertheless, the resulting Mg/Ca versus calcification temperature pair will be a realistic combination, and can be used in the calibration. Additionally, the position of the BMC shows significant variation over the year, so that it is reasonable to consider a seasonality effect as contributing further to the large scatter seen in the BMC core top database [e.g., Olson et al., 1988]. The location of core PS2495-3 is under open-ocean conditions at the Mid-Atlantic Ridge, rather than near the BMC, so that expatriation is not an issue for our downcore record.
3.2. Mg/Ca-Calcification Temperature Calibration
 Mg/Ca of G. inflata core top samples varies between 0.77 and 2.66 mmol/mol (Table 1). These ratios were combined with their respective calcification temperatures to derive the following exponential Mg/Ca-calcification temperature calibration equation (Figure 3):
with Mg/Ca in mmol/mol and δ18O-derived calcification temperatures in degrees Celsius. The regression curve is defined by the slope 0.076 (or A in general exponential regression curves) which is the temperature-sensitive component, and the y axis intercept 0.72 (or B in general exponential regression curves). The standard errors of the parameter estimates are ±0.006 for A and +0.045 and −0.042 for B.
 Apart from the small analytical errors which were presented in section 2.3., a significant uncertainty affecting the precision of temperature reconstructions based on equation (1) is related to the considerable spread in δ18O-derived calcification temperatures. We estimated an error of 2.25°C as the standard deviation from the residuals between calcification temperatures and temperatures calculated from equation (1). The standard deviation in temperature reconstructions based on equation (1) is significantly higher than error estimates for shallow-dwelling planktonic foraminifera of 1–1.5°C [e.g., Lea et al., 1999; Dekens et al., 2002; Anand et al., 2003] but in agreement with previously published calibrations for deep-dwelling foraminifera [McKenna and Prell, 2004; Cléroux et al., 2008; Regenberg et al., 2009]. This difference is most likely due to a combination of the following factors: (1) the variable depth range and, hence, conditions under which deep-dwelling planktonic foraminifera calcify; (2) the formation of a calcite crust typical for most deep-dwelling species, which will be present in different proportions depending on location or sample preservation; and (3) the uncertainties related to the estimation of apparent calcification depth (e.g., the choice of the paleotemperature equation used for δ18Oequ, influence of expatriation specifically at the location of the BMC, and possible foraminiferal δ18O-disequilibrium effects).
G. inflata Mg/Ca from gravity core PS2495-3 varies between 0.92 and 2.11 mmol/mol, which translates into a temperature range of between 2.9°C and 14.0°C (Figure 4). Terminations I and II are represented by large temperature changes of ∼8°C. Transitions from MIS5 to MIS4 and from MIS3 to MIS2 show sharp decreases in Mg/Ca from 1.43 to 0.95 mmol/mol and from 1.20 to 0.93 mmol/mol, respectively. These steps correspond to temperature changes of 5.5°C and 3.5°C, respectively. The G. inflata δ18O values range between 0.82‰ and 3.61‰ (Figure 4). Highest values occur during MIS2 and MIS4, when Mg/Ca is at a minimum. As temperature changes are as large as 8°C, the δ18O record is dominated by changes in temperature overruling changes in salinity. The Mg/Ca record and derived paleotemperatures will be used below in conjunction with an independent temperature estimator to test the feasibility of our calibration.
4.1. South Atlantic G. inflata Apparent Calcification Depth and Mg/Ca-Calcification Temperature Calibration
 The apparent calcification depth of G. inflata from the South Atlantic calculated for nonencrusted specimens from the 315–400 μm fraction is 387 ± 179 m. Plankton tow studies performed off southwest Africa [Lončarić et al., 2006; Wilke et al., 2006] showed that G. inflata occurs over a wide range of water depths with maximum abundance in the thermocline. Based on G. inflata δ18O values Lončarić et al.  showed that calcification also occurs over a large range of water depths with a mean apparent calcification depth of ∼250 m. Elderfield and Ganssen  assigned an apparent calcification depth for G. inflata of 300–400 m, based on core tops from the North Atlantic.
 In contrast to these findings, Cléroux et al.  showed after analyzing a collection of core tops from the North Atlantic that G. inflata mainly records conditions at the base of the seasonal thermocline (<100 m) and only descends deeper in the water column when temperatures at that depth are above 16°C. One explanation for this apparent discrepancy is that G. inflata occupies a different depth habitat in the North Atlantic in comparison with the South Atlantic [Bé and Tolderlund, 1971]. But, this does not explain the differences between the results of Cléroux et al.  and Elderfield and Ganssen  that are both based on North Atlantic samples. A potential explanation for this difference could be a different encrustation state of the analyzed specimens, which was not included in these studies. This apparent disagreement between different studies suggests the importance of defining a clear and narrow state of encrustation of the G. inflata specimens to be used for proxy analyses.
 Further comparison with Mg/Ca calibration equations for other deep-dwelling planktonic foraminifera, such as G. truncatulinoides and Globorotalia crassaformis, shows a roughly similar picture, although differences are present in absolute values, presumably pointing to interspecies differences and varying states of encrustation (Figure 3) [McKenna and Prell, 2004; Regenberg et al., 2009].
4.2. Potential Bias Caused by Different Size Fractions and States of Encrustation
 As G. inflata calcifies over a large depth range, Mg/Ca represents an average signal over this depth range. As the specimens descend through the water column they also acquire a calcite crust recording lower temperatures than the primary calcite. Hence, larger specimens are expected to contain a larger portion of calcite crust and lower Mg/Ca. Hathorne et al.  showed for G. inflata specimens from a North Atlantic sediment trap that Mg/Ca of the primary calcite is 2–3 times higher than the calcite crust. Cléroux et al. , on the other hand, analyzed two different size fractions of G. inflata, 250–315 μm and 355–400 μm, indicating that no significant difference in Mg/Ca was present between both size fractions.
 In this study we extended the range of size fractions to detect potential biases in Mg/Ca, though always selecting specimens with the same state of encrustation (defined as nonencrusted) as used for the calibration. Additionally, we also included samples with heavily encrusted, shiny specimens. An increase in size fraction is systematically related to a decrease in Mg/Ca (Figure 5). Mg/Ca in specimens <250 μm is warmer (2.0°C on average) than the nonencrusted 315–400 μm specimens. Mg/Ca from the 250–315 μm fraction (+0.7°C) is most similar to the 315–400 μm fraction, which is in agreement with Cléroux et al. . Lowest Mg/Ca is recorded by the largest specimens (>400 μm) and heavily encrusted specimens, which deviate 2.2°C and 4.4°C, respectively, from the fraction used for our calibration equation (315–400 μm). This experiment suggests that with increasing size the proportion of calcite crust increases, which was also shown for several other planktonic foraminifer species [Caron et al., 1990] indicating that the specimens calcified a larger fraction of the total test mass deeper in the water column. Therefore, their average chemical signature represents deeper conditions in the water column if compared to smaller, nonencrusted specimens. We state that careful and consistent selection concerning size and state of encrustation of specimens of G. inflata is essential for a reliable reconstruction of paleotemperatures.
 It is important to note that even though the temperature bias is calculated using our new Mg/Ca-calcification temperature calibration, that equation is not necessarily applicable to the other size fractions and encrusted specimens. Calcite crust was suggested to have a different temperature dependency than primary calcite [Bentov and Erez, 2006] and, therefore, when different portions of calcite crust are present also different temperature dependencies would apply.
4.3. Downcore Record of PS2495-3: A Test Case Application
 In order to illustrate the application of our Mg/Ca-calcification temperature calibration of G. inflata as a recorder of permanent thermocline temperatures we established a downcore Mg/Ca record spanning the last two glacial-interglacial cycles for core PS2495-3 in the central South Atlantic (Figure 1a). This site has been the focus of several paleoceanographic studies [Mackensen et al., 2001; Gersonde et al., 2003, 2004] (Figure 4) providing a well-dated stratigraphic framework.
 Presently, core PS2495-3 is located within the SAZ. Data from the World Ocean Atlas show that there is a temperature gradient between the SAZ and the PFZ of ∼5°C at 350–400 m water depth [Locarnini et al., 2006]. The reconstructed temperature change at core PS2495-3 over Termination I is ∼8°C (Figure 4). This value is similar to the one reconstructed for the sea surface based on foraminifera transfer functions [Gersonde et al., 2004]. Temperature reconstructions for midlatitude South Atlantic sites not under the influence of migrating oceanic fronts show changes in SST over Termination I of 2–4°C [Gersonde et al., 2003]. This suggests that an oceanic front migrated over our site during the Termination resulting in an additional 4–6°C temperature change. Therefore, our Mg/Ca temperature reconstruction shows that core PS2495-3 was located within the PFZ before Termination I and due to the southward migration of the SAF became under the influence of the SAZ at the end of Termination I.
 A marked difference between our record and the SST reconstructions from Gersonde et al.  is that Mg/Ca temperatures show a clearly warmer MIS3 in comparison with MIS2 and MIS4 (Figure 4). The modern gradient of ∼4°C between the sea surface and the permanent thermocline at our core site [Locarnini et al., 2006], which was also found for the Holocene, MIS2, and MIS4, is absent during MIS3. As our reconstruction of G. inflata apparent calcification depth is constant throughout the South Atlantic it seems unlikely that this can be explained by a change in habitat depth. A meridional vertical profile of the water column shows that midlatitudinal fronts are present down to a water depth of 400–500 m with the boundary between warmer and colder waters deepening toward the north resulting in the modern temperature gradient (Figure 1b). But when the surface and the permanent thermocline were bathed in the same water mass the water column would have been less stratified. This could possibly explain the similar temperatures reconstructed for the sea surface and for the permanent thermocline during MIS3. We suggest that during MIS2 and MIS4 the permanent thermocline at our site was under the influence of the PFZ, and the surface under influence of the SAZ, whereas both were bathed by the SAZ during MIS3.
 Reconstructed permanent thermocline temperatures for MIS5 and MIS6 are significantly warmer (∼4°C) than those reconstructed for the Holocene and MIS2, respectively (Figure 4). For MIS5 and MIS6 reconstructed temperatures based on Mg/Ca approach those reconstructed for the sea surface based on foraminifera transfer functions (Figure 4) [Gersonde et al., 2004], possibly suggesting a less stratified water column. We hypothesize that the warmer temperatures recorded for the permanent thermocline at core PS2495-3 during MIS5 are related to a stronger influence of the Subtropical Zone (STZ) if compared to the Holocene. Likewise, the PFZ would not have extended as far north during MIS6 if compared to its northernmost extension during MIS2, leaving the permanent thermocline at core PS2495-3 under the influence of significantly warmer waters of the SAZ. The warmer conditions during MIS5 and MIS6 in the permanent thermocline therefore seem to point to a more southward position of the SAZ in the permanent thermocline rather than large changes at the surface.
 The reconstructed temperatures for MIS2 and MIS4 appear close to or even lower than the lowest temperature tolerated by G. inflata of ∼3°C (Figure 5) [Bé and Hutson, 1977], and could have been caused by dissolution. Dissolution of biogenic carbonate in the water column or at the sediment-water interface preferentially dissolves higher-Mg portions of foraminiferal calcite [e.g., Brown and Elderfield, 1996]. As dissolution predominantly occurs in water masses undersaturated with respect to CO32− [Dekens et al., 2002; Regenberg et al., 2006; Mekik et al., 2007], deeper core locations are more easily affected by dissolution than shallower locations. At present core PS2495-3 is located in a water depth of only 3134 m and bathed by noncorrosive North Atlantic Deep Water and preservation is good. This is supported by Mg/Ca for G. inflata from a core top transect down to a water depth of 4000 m at the Rio Grande Rise which did not show any influence of dissolution [Mekik et al., 2010]. But, we cannot exclude that during glacial time periods more corrosive Antarctic Bottom Water had some influence at the site. Reconstruction of the calcite lysocline based on ultastructural investigations of the planktonic foraminifer G. bulloides showed increased influence of Antarctic water masses throughout the South Atlantic during glacial periods [Volbers and Henrich, 2004]. This led to a general shoaling of the calcite lysocline toward ∼3000 m water depth possibly causing some dissolution in our samples, and biasing Mg/Ca toward lower values. The correction of the Mg/Ca for dissolution would lead to an increase of 1–2°C [Dekens et al., 2002; Regenberg et al., 2006]. Thus, even corrected temperatures would still be significantly colder than MIS3.
 An alternative bias on the lowest temperatures of our downcore record could be related to the so-called cold-end effect of our calibration curve. This is a common feature of all Mg/Ca-temperature calibrations, both for planktonic and benthic foraminifera [Martin and Lea, 2002; Meland et al., 2006; Raitzsch et al., 2008]. Because small changes in Mg/Ca lead to large changes in temperature, slight differences in laboratory methods can have a significant effect on the reconstructed temperatures [Rosenthal et al., 2004]. However, considering the procedure at our laboratory as well as the occurrence of adjacent samples with low Mg/Ca during both MIS2 and MIS4, we consider that the cold-end effect is probably not significant and the reconstructed pattern of temperature change is therefore most likely representative.
 We established a Mg/Ca-calcification temperature calibration for the deep-dwelling planktonic foraminifer G. inflata for the South Atlantic (from 8°S to 49°S) based on a suite of 38 core top samples. Calcification temperatures were determined by comparing measured δ18O of G. inflata with equilibrium δ18O for calcite based on water column properties, resulting in an apparent calcification depth of 387 ± 179 m, reflecting permanent thermocline conditions, even under different upper water column structures. The resulting calibration equation is Mg/Ca = 0.72 ± 0.045/0.042 exp (0.076 ± 0.006 calcification temperature) (r2 = 0.81) and covers the temperature range from 3.1°C to 16.5°C.
 Additionally, we compared the Mg/Ca signal of several size fractions and different encrustation states to evaluate the bias introduced to paleotemperature reconstructions by an indiscriminate selection of specimens. Differences of up to 7°C between the different forms emphasize the importance of careful selection of specimens when using G. inflata for paleoceanographic reconstructions.
 We tested our new calibration on low-resolution gravity core PS2495-3 from the Mid-Atlantic Ridge raised at 41°S, which is within the present-day SAZ, covering the last two glacial-interglacial cycles. Paleotemperatures show large changes of up to 8°C at Terminations I and II, as well as changes of 4–5°C over the transition into and out of MIS3. These large changes suggest that the migration of the STF and SAF over the core site was responsible for 4–6°C of the total observed temperature change. Accordingly, the permanent thermocline at our site is suggested to have been located within the STZ (MIS5), the SAZ (MIS1; MIS3; MIS6), and the PFZ (MIS2; MIS4). The reconstruction largely fits with SST reconstruction for the same site based on foraminifer transfer functions. These results show that G. inflata Mg/Ca is a reliable proxy to reconstruct paleotemperatures at permanent thermocline depths, being particularly useful for the reconstruction of the migration of midlatitudinal fronts.
 We thank M. Segl, P. Witte, S. Pape, and M. Kölling for technical support; B. Donner, J. Bijma, and A. Mackensen for providing samples; E. Hathorne, M. Mohtadi, and S. Steinke for discussion; D. Heslop for statistical support; and four anonymous reviewers and the Editor for their constructive comments. This study was funded by the DFG-Research Center/Excellence Cluster “The Oceans in the Earth System” via a Marum Fellowship to J. Groeneveld and the CNPq-Brazil Fellowship granted to C. M. Chiessi.