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Corresponding author: V. Y. Ivanov, Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Ave., 105 EWRE, Ann Arbor, MI 48109, USA. (email@example.com)
 Large areas of Amazonian evergreen forest experience seasonal droughts extending for three or more months, yet show maximum rates of photosynthesis and evapotranspiration during dry intervals. This apparent resilience is belied by disproportionate mortality of the large trees in manipulations that reduce wet season rainfall, occurring after 2–3 years of treatment. The goal of this study is to characterize the mechanisms that produce these contrasting ecosystem responses. A mechanistic model is developed based on the ecohydrological framework of TIN (Triangulated Irregular Network)-based Real Time Integrated Basin Simulator + Vegetation Generator for Interactive Evolution (tRIBS+VEGGIE). The model is used to test the roles of deep roots and soil capillary flux to provide water to the forest during the dry season. Also examined is the importance of “root niche separation,” in which roots of overstory trees extend to depth, where during the dry season they use water stored from wet season precipitation, while roots of understory trees are concentrated in shallow layers that access dry season precipitation directly. Observational data from the Tapajós National Forest, Brazil, were used as meteorological forcing and provided comprehensive observational constraints on the model. Results strongly suggest that deep roots with root niche separation adaptations explain both the observed resilience during seasonal drought and the vulnerability of canopy-dominant trees to extended deficits of wet season rainfall. These mechanisms appear to provide an adaptive strategy that enhances productivity of the largest trees in the face of their disproportionate heat loads and water demand in the dry season. A sensitivity analysis exploring how wet season rainfall affects the stability of the rainforest system is presented.
 The role of the Amazon rainforests in the global cycles of carbon, water, and energy is well recognized and has been the subject of numerous studies [e.g., Salati and Vose, 1984; Schlesinger, 1997; Davidson et al., 2012]. What emerges from much of the research is that the productivity and function of these ecosystems among other factors are profoundly related to climatic variability of precipitation. While the mean annual rainfall over the Amazon basin is ≈ 2100 mm [Marengo, 2004], about half of the area [Sombroek, 2001; Xiao et al., 2006] experiences a pronounced drought season defined as months with less than 100 mm total precipitation [Shuttleworth, 1988]. As demonstrated by Fatichi et al. , globally, interannual variability of precipitation is strongly related to precipitation intraannual seasonality; seasonally dry areas of the Amazon are thus statistically likely to experience higher fluctuations of yearly rainfall, as compared to areas with aseasonal rainfall.
 The pronounced rainy and dry seasons, particularly in the central and eastern Amazon Basin [e.g., Sombroek, 2001; Malhi and Wright, 2004; Xiao et al., 2006], have significant implications for the system function [Brando et al., 2010; Davidson et al., 2012]. It is a matter of significant scientific concern to identify to what degree the Amazon rainforests are vulnerable to droughts. As recent research indicates, the understanding of drought effects and essential controlling factors of the rainforest function are yet to be fully understood.
 The evidence available to support or contradict each of these hypotheses is variable. First, in the case of maximum rooting depth, the presence of roots at depths > 10 m is clear, but their function remains quite uncertain, and their importance cannot be confirmed or denied as yet. The open questions are how deeply roots can really grow, what kind of hydraulic limitations exist on the transfer of water from deep locations to the top of canopy, and whether there are sufficient roots at deep locations. For example, Nepstad et al.  estimated that only about 10% of the total rooting mass of a forest was at depths between 4 and 10 m. In a modeling study, Grant et al. demonstrated that a root system depth of only 8 m was needed to avoid water limitations for a 5-month dry season, but the generality of such an inference remains uncertain. Second, the phenomenon of hydraulic redistribution (i.e., the process of water transfer by roots following the countergradient of soil water potential, most efficient when transpiration uptake is negligible) has been well documented for arid and temperate climate plant species (e.g., for a review seeCaldwell et al. ) and has been demonstrated for three tree species in the Tapajós National Forest, Brazil [de Oliveira et al., 2005]. One may note that de Oliveira et al.  observed sap flow reversals; whether this is a sufficiently strong argument for advocating the importance of hydraulic redistribution for the entire ecosystem function [e.g., Lee et al., 2005] remains uncertain. Other field and modeling experiments postulate that, in fact, the mechanism of hydraulic redistribution cannot contribute significantly to the soil water dynamics [e.g., Romero-Saltos et al., 2005; Markewitz et al., 2010]. A conclusion is that further empirical evidence is needed to assess whether hydrologically significant amounts of water can be transferred and deposited in soil-surrounding roots. So far, quantitative assessments for the Amazon region come from modeling studies that yield estimates “on the upper end of the spectrum” [e.g.,Neumann and Cardon, 2012], as compared to most laboratory and in situ measurements. Third, a similar comment concerns the mechanism of water uptake by leaves: further, quantitative empirical evidence is needed to confirm the plausibility of its significance. Lastly, a recent claim of possibly predominant groundwater effects [Fan and Miguez-Macho, 2010] cannot be supported by field observations at some of the “benchmark” sites [e.g., Goulden et al., 2004; Hutyra et al., 2007; Nepstad et al., 2002]: They present no evidence of shallow water table. The question of the exact water-stress avoidance mechanism has therefore not been fully resolved. Other explanations should be attempted that will help focus future observational campaigns, so that “unfit hypotheses” [Popper, 1972] can be objectively evaluated.
 This study makes a further effort to study the hydrology of an Amazonian rainforest. A vegetation-hydrology model that parameterizes the essential canopy-soil water-energy processes using a simplified three-big-leaf representation of canopy vertical structure is developed. The representation of a very deep (∼36 m) soil profile allows the model to explicitly resolve the propagation of wetting and drying cycles into the soil column underlying forest vegetation. Comprehensive observational data from a flux tower site in Tapajós National Forest, Brazil, are used in this study. Meteorological data from the tower for the period of 2002–2005 serve as the model forcing. Observed canopy phenology, energy fluxes, soil texture and water retention properties, and profiles of root biomass are used to parameterize and constrain the model performance. A set of numerical experiments has been designed to address (1) depth of the rainforest root system as the only strategy for stress avoidance; (2) the capillary properties of clayey Oxisol soils that could possibly lead to the upward flow of water from soil layers below the deepest extent of roots; specifically, if a sufficiently high gradient of soil matric potential is created in the root zone because of moisture uptake by plants, a flow in the direction opposite to the gravitational force may take place; and (3) the existence of specific water uptake niches in the soil column (i.e., substantially different depths of centroids of moisture uptake profiles) corresponding to root systems of trees located at different levels in the vertically structured canopy, i.e., overstory, midsize, and understory trees. These several possible explanations of the water-stress avoidance paradigm either have not been considered in sufficient detail in models or have remained entirely unexamined. Neither hydraulic redistribution nor leaf water uptake is addressed in this model application, and the role of groundwater is eliminated by the choice of site (seesection 2.1).
 One of the indirect facts pointing to the plausibility of the hypothesis referring to the existence of water uptake niches is the remarkable agreement of the main outcomes of two long-term rainfall exclusion experiments described byNepstad et al. [2002, 2007] and da Costa et al. as well as a pan-tropical assessment of drought-related tree mortality byPhillips et al. , and, to some extent, observations for a neotropical forest system by Condit et al. : in all studies, there was an asymmetric response to prolonged droughts by overstory and understory trees. Specifically, large trees were consistently found to be the most vulnerable. Synthetic numerical experiments augmenting this study mimic the drying signal of rainfall exclusion experiments and consistently with empirical observations reproduce an asymmetric response by large and small trees in conditions of root niche separation. A subsequent analysis demonstrates a relative sensitivity of study inferences with respect to the key parameters quantifying plant water limitations. Overall, this study is best described as a model-based analysis of the plausibility of various mechanisms, attempting to address a wide spectrum of empirical observations.
2. Data and Methods
2.1. Site Description
 Data are from the flux tower site in Tapajós National Forest (Brazil) (2°51.4′S, 54°57.5′W) near km 67 (referred to as “km 67” throughout) of the Santarém-Cuiabá highway BR-163. The site was a part of the large-scale biosphere-atmosphere experiment in Amazonia (LBA-ECO) and has been used in a number of studies described in previous publications [e.g.,Hutyra et al., 2007; Rice et al., 2004; Vieira et al., 2004; Saleska et al., 2003]. Additionally, a large throughfall exclusion experiment was established in the relative proximity of the flux tower (∼5 km) in 2000 [Nepstad et al., 2002], which monitored a variety of ecosystem characteristics in the 1-ha treatment and control plots [e.g.,Brando et al., 2008; Nepstad et al., 2007]. These studies provide a complete description of different system characteristics, and the reader is referred to the cited papers for details. The region has a mean annual temperature of 25°C with a mean annual precipitation of ∼2000 mm, variable between 600 and 3000 mm yr−1 [Nepstad et al., 2002]. The area is subjected to a seasonal drought, i.e., months with less than 100 mm precipitation, with a mean span of 5 months, typically lasting from 15 July to 15 December [Parrota et al., 1995]. The water table is very deep, ∼100 m below the soil surface [Nepstad et al., 2002]. Soils are clay-rich, deeply weathered Oxisols. Patches of higher sand content (Santarém Ultisols) occur on slopes and in topographic lows of the surrounding area but not at the study site. The forest is located on flat terrain, an erosional remnant plateau, with a limited drainage network formed on sediments of the Barreiras formation [Silver et al., 2000]. The forest has a closed canopy with a mean height of approximately 40–45 m and emergent trees reaching up to 55 m. The vertical canopy distribution is stratified [Vieira et al., 2004] with a fairly distinct three-level structure that represent the highest, midrange, and smallest trees. The forest at the site exhibits a robust increase of latent heat flux during dry seasons (for details, seeHutyra et al. ), responding to increased light levels (Figure S1a in the auxiliary material). The annual variation of foliage leaf area index (LAI) is within 10% of the maximum value, with the maximum apparently out of phase with the radiation cycle (Figure S1b in the auxiliary material and Brando et al. ). Domingues et al.  showed that the leaf density was approximately constant throughout the canopy profile, with about 35% of leaf area attributed to the top 10 m of the canopy.
2.2. Hydrometeorological Forcing Data
 Gap-filled meteorological data for the period of January 2002 to January 2006 are used as atmospheric forcing to the model. Specifically, the hourly time series of the following variables observed at km 67 [Hutyra et al., 2007] serve as the model input: hourly precipitation (measured at 42.6 m above the forest floor), air temperature (57.8 m), water vapor partial pressure (computed from vapor molar concentration measured at 62.2 m), wind speed (57.8 m), CO2partial pressure (measured as molar concentration at 62.2 m), atmospheric pressure (ground level), and incoming short-wave and long-wave radiation fluxes. Data gaps were fairly insignificant (1%–9% of the series) for most of the variables and were filled with the mean monthly values corresponding to the hour of the day with the missing value. Only radiation data contained a large fraction of gaps (∼35%–55% of the series), and thus several sources/methods were used to complete the series. The gap-filling procedure is outlined in theauxiliary material (section S.1).
2.3. Soil Hydraulic Properties
 The soils at the km 67 site study are clayey Oxisols that are deeply weathered with no concretions or impeding layers, at least in the upper 12 m [Nepstad et al., 2007]. The van Genuchten-Mualem soil hydraulic model [van Genuchten, 1980] was chosen to describe the dependence of conductivity and soil matric pressure on moisture content. Due to the absence of detailed, on-site measurements of soil hydraulic properties as well as uncertainty associated with each indirect method, several approaches and data sources were used to infer the saturated conductivity and soil water retention parameters. Previously reported soil water retention parameters byBelk et al.  did not favorably compare with the other independently obtained parameterizations (see sections 2.3.2–2.3.4 and Figure 1) and therefore were not used. Note that the generated ensemble of soil parameterizations permits addressing robustness of study inferences with respect to uncertain properties of the soil. Following sections describe the methodology used in obtaining the parameter values.
2.3.1. Saturated Hydraulic Conductivity
 Values of the saturated hydraulic conductivity were digitized from the manuscript of Belk et al. , who measured conductivity over the upper 4 m of the soil profile. Geometric averaging of depth-interpolated values was carried out to obtain for the 0.05–0.3 m depth range and for the 0.3–4.0 m depth range. These values were used in the two scenarios described in sections 2.3.2 and 2.3.3.
2.3.2. Measured Soil Water Retention Data
 The soil water retention data were obtained by the authors in 2007 (unpublished data set). Soil samples were collected at a location that is in relative proximity to km 67 (within ∼1 km radius); however, the exact location was not recorded. Laboratory measurements of water retention properties were carried out for the following soil matric pressures: 6, 10, 33, 100, and 1500 kPa. Samples were taken from the following depths: 5, 15, 30, 50, 100, and 200 cm. Due to significant differences in the soil water retention data between the surface layer and deeper layers, the measurements were grouped according to the following depth ranges: 0.05–0.3 m (what is referred to as “CO-surface” group) and 0.5–2 m (“CO-deep” group). These two sets of parameters define the “CO” soil hydraulic parameterization. A nonlinear optimization of parameter values of thevan Genuchten  model was subsequently carried out. The data and the fitted water retention curves for the two depth ranges are shown in Figure 1. The corresponding parameter values are provided in Table 1.
Table 1. Soil Hydraulica, Heat Transfer and Albedob Parameters for Different Soil Parameterization Scenarios
Ksn is the saturated hydraulic conductivity in the normal to the soil's surface direction, is the saturation moisture content, is the residual moisture content, n and αare the fit parameters of the van Genuchten-Mualem soil hydraulic model [van Genuchten, 1980]. The soil albedo and heat transfer parameters are assumed to be uniform for all soil parameterizations.
Since undercanopy soil is assumed to be a Lambertian surface, albedos for direct beam, , and diffuse, , shortwave radiation fluxes are equal. The values of the shortwave albedos for saturated soil ( ) are 0.11 for the visible and 0.225 for the near-infrared spectral bands, respectively [Ivanov et al., 2008]. The values of the shortwave albedos for dry soil ( ) are 0.22 for the visible and 0.45 for the near-infrared spectral bands, respectively. The soil heat transfer properties are assigned the following values: and are the dry and saturated soil thermal conductivities, respectively, and is the heat capacity of the soil solid [Ivanov et al., 2008].
See section 2.3 for the definitions of soil parameterization scenarios.
2.3.3. Parameter Estimation Using Pedotransfer Functions
Tomasella et al.  developed generic pedotransfer functions for South American soils, allowing one to derive the parameter values of the van Genuchten  model based on soil textural and chemical composition. Field soil texture data were obtained by the authors in 2003 (unpublished data set): the surface soil (top 10 cm) composition was reported as 91% clay, 7.8% silt, and 1.2% sand. The organic carbon content was reported as 2.68 , and soil bulk density was measured as 1.0225 . As the fraction of sand that was fine was unknown, it was assumed to be either 0%, 50%, or 100%. Using the Tomasella et al.  functions, three parameter sets were estimated assuming these fractions. The obtained parameter sets differed only slightly, and thus they were geometrically averaged to obtain a single parameter set. The derived water retention curve representative of the entire soil column is shown by the Tomasella et al. (2000) curve in Figure 1. The corresponding parameter values are provided in Table 1 as the “TH” parameterization.
2.3.4. Inverse Parameter Estimation
 An inverse method of Hou and Rubin was used to derive soil properties using soil water dynamics data resolved at multiple depths. The only available high-frequency, long-term record of soil moisture data was available for a site at km 83 of the Santarém-Cuiabá highway [Bruno et al., 2006]. This data set was used in the inverse estimation procedure. Eight cases were selected corresponding to 10–11 h nighttime intervals with zero or insignificant observed evaporation. The cases included periods with the highest and lowest observed soil moisture, as well as the cases with the highest moisture variability in the 10 m soil profile. Several attempts to derive layer differences yielded posterior distributions that were identical to prior distributions. Therefore, the final search aimed to estimate hydraulic properties representative of the entire soil column. The derived water retention curve is shown in Figure 1. The depth-uniform saturated hydraulic conductivity was estimated to be . The corresponding parameter values are provided in Table 1 as the “MRE” parameterization.
2.4. Root Profiles
 Data on the distributions of root biomass density were obtained as one of the outcomes of the throughfall exclusion experiment [Nepstad et al., 2002] that was carried out in the relative proximity of the flux tower (∼5 km). For the treatment site, only data obtained before the beginning of the experiment were used. Observations of fine root biomass were used (a fine root has the diameter smaller than 2 mm) assuming the suberized fraction of a fine root is small and almost the entire root surface can be used for plant moisture uptake [Taiz and Zeiger, 2006, p. 56]. Fine root distributions were therefore associated with uptake profiles. A “generalized” root profile was created based on the actual measurement data shown in Figure 2a. Two scenarios of maximum root depth were considered: m and m. The procedure resulting in these magnitudes is described in the auxiliary material, section S.2. The corresponding root profiles were used in the modeling efforts (section 2.5.4).
2.5. Modeling Efforts
 An ecohydrological model that parameterizes essential canopy-soil water-energy processes using a three-big-leaf representation of canopy vertical structure and a finely resolved deep soil profile was developed in this study. As is the case with any modeling effort, a number of simplifying assumptions were made in the representation of structural elements of the vegetation-hydrology system, its intra- and interannual dynamics, as well as in the description of relevant physical processes. These are outlined in the following.
2.5.1. Ecohydrological Model
 An ecohydrologic model [Ivanov et al., 2008], TIN (Triangulated Irregular Network)-based Real Time Integrated Basin Simulator + Vegetation Generator for Interactive Evolution (tRIBS+VEGGIE), is used in this study. The model mimics principal water and energy processes over the complex topography of a river basin and links them to essential plant biochemical processes and phenology. The model design emphasizes dynamic interactions between vegetation system and subsurface hydrological dynamics. Each computational element exhibits a “big-leaf” representation of the canopy coupled to a multilayer soil-root model that computes soil moisture and heat transport and root water uptake.
 Because topography within the tower footprint is flat (section 2.1) and relevant observations on canopy dynamics (section 2.5.3) were available, the model application was “reduced.” Specifically, the model was applied at the plot scale, and vegetation dynamics were not explicitly simulated. Only the biochemical model of canopy stomatal behavior [Farquhar et al., 1980; Collatz et al., 1991] was used to simulate the response of latent heat flux to the ambient environment. The amount of leaf area as well as structural characteristics of vegetation were imposed as a predetermined model input (see section 2.5.3). Furthermore, the model formulation described in Ivanov et al.  has been substantially modified to adapt the model to conditions of the study site that exhibits complex canopy structure and deep soils. These changes are briefly outlined in the auxiliary material, sections S.3 and S.4. For the reader's convenience, Appendix A provides the formulation of a heuristic soil moisture availability factor, , which is frequently used throughout the text. In short, the formulation of the lumped factor is based on widely used relationships [Bonan, 1996; Feddes et al., 2001] that parameterize a decrease of water availability with drying soil conditions. The factor is used to regulate the stomatal conductance when transpiration flux is computed and its departure from unity indicates soil control on the flux; can be interpreted as a metric of water stress. Table 2 contains the parameter values used in this study. The notation used for the parameters is the same as that of Ivanov et al. .
Table 2. Vegetation Biophysical, Photosynthesis, Interception, and Water Uptake Parameters Used in the Modela
dleaf (m) is the mean leaf size; is the parameter of departure of leaf angles from a random distribution; and (−) are the leaf reflectances and transmittances, respectively; and (−) are the stem reflectances and transmittances, respectively; “VIS” and “NIR” are used to denote the visible and near-infrared spectral bands, respectively; ( CO2 m−2 s−1) is the maximum catalytic capacity of Rubisco at 25°C; (−) is the time-mean photosynthetically active radiation (PAR) extinction coefficient used to parameterize a decay of nitrogen content in the canopy;m (−) is an empirical parameter used as a slope factor in a linear model relating the net assimilation rate and stomatal conductance; b is the minimum stomatal conductance; ( CO2 μ ) is the intrinsic quantum efficiency of CO2 uptake; Kc ( ) is the canopy water drainage rate coefficient; gc ( ) is the exponent parameter of canopy water drainage rate; (MPa) is the soil water potential at which stomatal closure begins; and (MPa) is the soil water potential at which plant wilting begins.
Water Uptake Parameters
2.5.2. Soil Profile
 In representing the soil profile in the model, two conditions had to be accounted for: (1) the capability to incorporate the deepest root profile with m and (2) the need of providing a sufficient soil buffer under the root zone to alleviate the effect of an assumed free drainage boundary condition at the bottom of the soil column. With respect to the latter, the intention was to create a soil buffer that could generate upward unsaturated flow if sufficiently high tensions developed in the root zone because of moisture uptake by roots. Consequently, a deep, 36 m soil profile was used to model the subsurface soil moisture dynamics. A regular mesh resolution was selected to be 30 mm, which resulted in 1200 computational nodes.
2.5.3. Above-Ground Vegetation Components
 A three-big-leaf representation of the forest canopy was developed in an attempt to represent the vertical structure of the canopy, which has been observed to be distinctly stratified [Vieira et al., 2004]. Rice et al. describe “emergent,” “canopy,” “subcanopy,” and “suppressed” trees. In this study, the “top-canopy” (overstory) trees represent the emergent and canopy trees; the “mid-canopy” and “bottom-canopy” (understory) trees, respectively, represent subcanopy and suppressed trees. These types of trees were assumed to be sufficiently different in their biophysical and biochemical properties, for example, reflecting observed changes in the characteristics of sunlit and shaded plants [e.g.,Taiz and Zeiger, 2006]. The outcome of such an assumption is that different canopy levels represented different tree functional types, even though some of the same species are likely to be present in each canopy level. Note the interchangeable use of “tree type” and “canopy layer” in the following text. Details of treatment of canopy layers are presented in the auxiliary material, section S.3; the parameter values are provided in Table 2.
 The amount of leaf area as well as structural characteristics of vegetation were imposed as predetermined model input. The data on canopy dynamics were obtained from the database of the LBA experiment in Amazonia (http://lba.cptec.inpe.br). This data set contains measurements of total LAI obtained at monthly intervals in the control plots of the rainfall exclusion experiment [Nepstad et al., 2002]. The LAI data span the period of 2000–2004. According to Domingues et al. , the leaf density is approximately constant throughout the canopy profile at the site; consequently, the mean annual cycle of LAI for each of the “big leaves” was obtained by dividing the total LAI (auxiliary material, Figure S1b) by three.
 The stem area index (SAI) [e.g., Bonan, 1996] was assumed to be 0.2 for each of the tree types. Each canopy layer was assumed to be uniformly occupying the entire plot area, i.e., the vegetation fraction was set to one for each of the tree types. This apparent simplification neglects the spatial heterogeneity of the canopy, i.e., the existence of gaps as well as patches with LAI higher than the one assumed. Remote-sensing data that permit the inverse estimation of LAI, e.g., the data from moderate resolution imaging spectroradiometer (MODIS;http://modis.gsfc.nasa.gov, Myneni et al. ), do not offer sufficient resolution and quality to assess the degree of such variability at the study site. The importance of representation of canopy heterogeneity at the tree scale is hard to assess since relevant studies are still in their nascence [e.g., Bohrer et al., 2009].
2.5.4. Below-Ground Vegetation Components
 Each of the tree types defined above according to the canopy level (section 2.5.3) contributes to the distribution of fine root biomass described in section 2.4. A first-order assumption one can make is that each of the types contributes equally to the observed density at any depthz and therefore root density for any tree type can be obtained as the total root biomass divided by three. For each of the considered maximum root depths, ZRootD and ZRootS, this serves as the “control” scenario (Figure 2b) against which all other root distribution scenarios are compared.
 Certainly, the control root distribution case is only one plausible scenario of the shape of individual profiles corresponding to trees that have different positions in the canopy. Because it is practically impossible to quantify the distribution of root biomass of a given tree type in the bulk root biomass from in situ observations (and to the author's knowledge, there are no available generic methodologies), this study has generated an ensemble of individual root distributions. The developed ad hoc partition procedure (see the auxiliary material, section S.5) yielded 12 additional permutations of individual root profiles that are illustrated in Figure 2b for the maximum root depth ZRootD. As the number of permutation scenario increases from 1 (the control) to 13, the profiles become more different from the control root scenario and trees of the upper-canopy level contain progressively higher fraction of roots at deeper locations (Table 3), while trees of the bottom-canopy layer contain higher fraction of roots at shallower soil layers. Note that this also implies varying root depths for all scenarios other than the control one. For example, asTable 3illustrates, in the case of the largest difference among the profiles, i.e., the root scenario 13 referred to thereafter as the “scenario with distinct niches,” the roots of understory trees extend only to ∼2.01 m, the root depth of mid-canopy trees is ∼3.03 m, while the roots of overstory trees occupy the entire depthZRootD (30.2 m).
Table 3. An Illustration of Hypothesized Root Fractions Contained in Different Soil Layers for Trees Occupying Particular Canopy Levelsa
Root Fraction 0–30 cm (%)
Root Fraction 30–60 cm (%)
Root Fraction 60–100 cm (%)
Root Fraction 100 cm - Root Depth (%)
Root Depth (cm)
Two maximum root depths, ZRootS and ZRootD, are used. Note that the values reported were obtained in relation to the total hypothesized biomass within the root depth (last column of the table), i.e., a small fraction for a deep root zone can imply a large absolute surface area. The actual spatial resolution of the subsurface model is 30 mm; the values shown were obtained by lumping root fractions for specified soil layers.
Top-, mid-, bottom-canopy
distinct niches scenario
Top-, mid-, bottom-canopy
distinct niches scenario
 The depth m was considered in the design to represent another choice of the maximum possible root depth. Thirteen root permutations were generated by terminating the individual profiles shown in Figure 2b at ZRootS to exclude roots beyond that depth. Similar to the scenarios for ZRootD, scenario 1 among these permutations represents the control and scenario 13 corresponds to the scenario with distinct niches (Table 3).
 Overall, 26 root scenarios (the control scenario plus 12 permutations of individual root profiles for the two maximum root depths ZRootD and ZRootS) for each soil type were generated. In total, this resulted in 78 simulation scenarios.
 The ecohydrology model requires the input of a potential strength of moisture sink in each subsurface mesh node [Ivanov et al., 2008]. Such a profile is obtained by computing the fraction of roots contained in a control volume of each mesh node with respect to the total root biomass.
2.5.5. Soil Moisture Initialization
 The deep soil profile of a fine-textured soil may exhibit significant memory effects due to the persistence of soil moisture initial conditions in simulations. This study attempted to minimize such adverse initialization impacts by using a model “spin-up.” Specifically, each scenario of maximum root depth (ZRoot) for each soil type was simulated with 20 years of meteorological forcing obtained as five 4-year cycles of the original forcing data, January 2002 to January 2006. The soil matric head profile at the end of each of these spin-up simulations was used to initialize simulations for the corresponding combination of soil type and root depth.
3.1. Model Calibration
 The tRIBS+VEGGIE model does not have an automated parameter calibration routine. Manual model calibration was carried out with the main objective to match the observed daily and seasonal cycles of net radiation, latent and sensible heat fluxes.
 Because the study addresses the effects of dry periods on vegetation water uptake, the soil hydraulic parameters (Table 1) and the root fractions specifying the strength of transpiration moisture sinks (see Appendix A, equation (A2)) should also be considered as unknown parameters. An arbitrary choice of soil type and root distribution would be unjustified because both can strongly affect the dynamics of plant water limitations. Therefore, the calibration strategy was based on the assumption that forest does not experience any water stress during drought periods of the considered simulation period. Empirical evidence of increased forest greenness and sustained evapotranspiration fluxes during prolonged dry periods [e.g., Huete et al., 2006; Hutyra et al., 2007; Myneni et al., 2007; Saleska et al., 2007] provide some support for such an assumption. During calibration, water stress for each tree type was artificially set to zero at each computational step and, consequently, calibration was soil-type and root-distribution independent. As a result, latent heat flux, simulated accounting for the energy constraints (auxiliary material, section S.4, equations (S-10)–(S-12)), essentially represented “potential evapotranspiration.”
 Note that the total observed daytime sensible and latent heat fluxes are lower than the observed net radiation: by 21.5% during wet season and 19.5% during dry season periods (see a detailed discussion in Hutyra et al. ). This substantially complicates the definition of an exact energy partition. Because net radiation was believed to be measured more precisely than heat fluxes, its accurate simulation at the daily and seasonal scales was specifically targeted. The simulated net radiation thus matched observations nearly perfectly, as will be demonstrated in section 3.4.
 By design, the model conserves energy and therefore any partition of simulated net radiation into heat fluxes cannot compare favorably with the measured values because of the aforementioned 19.5%–21.5% heat imbalance in observational data. This “surplus” heat energy was distributed among the fluxes of latent, sensible, and ground heat and fluxes going into storage (heat exchanged by precipitation was not accounted for, see Hutyra et al.  for further statement of issues related to seasonal heat budget closure). To some extent, the final outcome of calibration is therefore affected by this subjective partition, but it is difficult to assess its exact impact. Several additional simulations were carried out in which parameters were redefined, so as to make either latent or sensible heat receive a higher fraction of the “surplus” energy (not shown). It was inferred that the main conclusions of this study hold, regardless of the decision made in calibration.
 Note that the assumption of potential evapotranspiration used in the calibration process aligns well with the concept of water stress avoidance emphasized by the study. The experimental design then allows the exploration of scenarios that can yield fluxes of nearly the same magnitudes as the rates obtained during calibration. The exact implication of assuming the observed fluxes to be potential evapotranspiration is hard to assess; however, a lack of clear water stress signals in the data of Hutyra et al. and the “back-of-the-envelope” estimates implies that the errors cannot be large and affect major inferences of the study.
 All of the model parameters used in the description of energy fluxes are provided in Table 2. Among these, the parameters of the “interception” and “water uptake” groups were not modified. Most of the “biophysical” parameters were assigned based on values reported in literature [e.g., Bonan, 2008]; only and (visible band) were slightly modified to represent the properties of vegetation acclimation to different vertical positions in the canopy. Using the same considerations, only and parameters were calibrated in the “biophysical” group.
3.2. Annual Cycle of Evapotranspiration and Water Stress: Identical Root Distributions
 The annual cycle of evapotranspiration obtained in the calibration procedure is illustrated in Figure 3a, as the “no stress” scenario, which is qualitatively similar to observations. Figure 3 presents the simulation results as averaged annual cycles of evapotranspiration components computed for the three soil scenarios under the assumption of the identical root fractional distribution (i.e., the “control” distribution, see section 2.5.4 and Figure 2b). Specifically, the maximum root depth was set to either ZRootD or ZRootS, and all tree types were assigned to have the identical root profile, which is the control root scenario (see Figure 2b). Under the three soil (“CO,” “TH,” “MRE”) scenarios and the control root scenario, Figure 2b further illustrates that the model exhibits poor performance during dry seasons, i.e., a depressed flux of total evapotranspiration is simulated, as compared to observations (particularly, for the ZRootS scenarios). Evaporation from soil and canopy interception storage shows no sensitivity to any of the soil or root scenarios (Figures 3a and 3b). The poor model performance during August through December period results from a dampening of the transpiration flux as the dry season progresses. The simulations with ZRootS exhibit highest sensitivity for all soil types. Also note that the “no stress” simulation exhibits higher cumulative evapotranspiration than the observed series. This is due to the aforementioned issue of energy imbalance (section 3.1) and the calibration objective to achieve accurate simulation of net radiation at the daily and seasonal scales. The procedure yielded a particular partition of net radiation with simulated evapotranspiration exceeding observed values by ∼4.5%.
Figure 3c illustrates the cycles of the soil moisture availability factor (see Appendix A), , computed as the average for the three tree types. The departure of from unity indicates soil control on the transpiration flux and water limitation experienced by vegetation, i.e., can be interpreted as a metric of water stress. As seen in the figure, all of the soil-root scenarios resulted in some degree of water stress experienced during dry periods. However, the average stress is fairly small for the m root scenario. The latter result implies that having (very) deep roots may be a sufficient strategy for avoiding the drought stress; i.e., in this case, the annual root water uptake is distributed over a larger soil depth, providing access to a larger water reservoir and making the uptake density smaller per unit depth.
3.3. Can Niches of Water Uptake Sustain Transpiration?
 In addition to the “control” root profile, 12 permutations of individual root profiles were used for the two maximum root depths, ZRootD and ZRootS, resulting in 26 root scenarios for each soil type (78 scenarios in total). Figure 4a illustrates the factor computed as the time-average value for a particular permutation scenario of root distribution (also averaged across the tree types). As seen, the more vertically “segregated” the profiles become (i.e., the higher the root scenario number), the less overall stress is reproduced by the model. The shallower root depth scenarios exhibit substantially higher sensitivity to root permutations than deeper root depth scenarios. Note that a comparison of absolute magnitudes of evapotranspiration is biased: higher (as compared to observations) latent heat fluxes were simulated intentionally because of the energy imbalance in the observed data (section 3.1).
Figure 4bshows the pattern of the root-mean-square error of mean daily evapotranspiration (RMSEET) with respect to the observed magnitudes. The behavior of RMSEET is not as consistent as that of , i.e., the increase in the root scenario number does not lead to a continuously smaller RMSEET. This can be explained by the fact that, overall, the model generally overestimates evapotranspiration flux (at maximum, by ∼4.5%; section 3.2). The higher implies even larger evapotranspiration magnitudes, which led to a somewhat poorer comparison of the simulation results in terms of RMSEET for larger numbers of root scenario.
Figures 4c and 4d interpret the patterns of and RMSEET behavior with respect to the root scenarios in terms of times series of evapotranspiration flux. The “TH” soil type is used that exhibited the highest water stress for the “control” root profile (Figure 3c) as well as the highest sensitivity of results to the permutations of tree root profiles (Figure 4a). As the root scenario number increases, indicating the growing degree of difference in the root fractional distributions, the simulated water stress during dry seasons vanishes. This is particularly pronounced for the shallower root scenarios (Figure 4d).
 As the center of mass of root fractional distribution changes (increasing permutation number), so does the interrelated effect of moisture uptake because of the continuity of the soil water profile. In other words, trees taking up moisture in a particular region of the soil profile necessarily impact uptake and drainage characteristics in other soil layers. The temporal evolution of the soil moisture profile for the two root scenarios corresponding to the maximum root depth ZRootS, the identical, control root scenario and scenario with distinct niches, are shown in Figure 5. Figure 5 clearly shows that the scenario with distinct root niches avoids the extreme seasonal drying of the upper soil layer, where the control root scenario assumes the location of the centers of root mass for all trees. The scenario with distinct root niches leads to a less variable distribution of the drying signal with depth. It can be generally inferred that the variance of soil moisture profile over the root zone depth during dry seasons is smaller for the higher root scenario number (not shown). It can also be concluded that there is no significant effect of root distribution on the soil moisture dynamics during wet periods, except for the year of 2003 that had an unusually low wet season rainfall. By wetting the deep soil profile down to 36 m (not shown), wet season precipitation appears to have a “resetting” effect on soil water in other years. Drying season starts with a fairly uniform profile of moisture near 0.55–0.57 volumetric content.
3.4. Seasonality of Energy Fluxes
Figure 6 illustrates the observed and simulated diurnal cycles of energy fluxes. The results correspond to the “CO” soil parameterization, root scenario 6 that did not exhibit appreciable water stress, and thus the results are also representative of calibration (see section 3.1).
 The observed cycle of net radiation is almost perfectly reproduced in the simulation results; however, the simulated heat cycles exhibit certain differences from observations. As pointed out in section 3.1, one of the principal difficulties in objective evaluation of such a comparison is the fact that measurements of latent and sensible heat fluxes do not balance the observed net radiation by about 20%. Since the modeled fluxes have to conserve net radiation exactly, the difference with observations was subjectively distributed among the different flux terms. The inclusion of ground heat (small) and storage (auxiliary material, section S.3) terms helps in balancing the net radiative flux, but the overall partition does not appear to be most appropriate. For example, sensible heat flux is generally “overestimated” throughout the day; the latent heat flux is “underestimated” in the first half of the day but is overestimated during the second half. The underestimation effect is partially due to the inclusion of the heat storage term; yet, the net effect is that the total evapotranspiration is somewhat larger than observed, particularly during dry seasons (see Figure 3a). Figures 6c and 6dillustrate that daytime, above-canopy heat fluxes are truly dominated by fluxes associated with vegetation.
 The computed temperatures of canopy layers and undercanopy ground (affecting the energy partition shown in Figure 6) are qualitatively consistent (see the auxiliary material, Figure S2): the top-canopy layer exhibits highest temperatures during the daylight hours and the lowest temperatures during nighttime hours; middle- and bottom-canopy layers show progressively smaller diurnal temperature variability and are somewhat warmer than the above-canopy air temperature throughout the day; ground surface temperature has the lowest diurnal variability and exhibits cooler temperatures during daylight hours because of low levels of radiation reaching the forest floor. The partition of the total-canopy layer latent heat exhibits a peculiar feature of the system: despite the assumed same LAI for all canopy layers, the top-canopy level contributes by far the highest evapotranspiration flux, with lower canopy levels contributing progressively smaller fluxes. Such a feature is apparently related to the substantially higher light levels that are simulated for the top canopy, and, to a lesser extent, to the assigned distribution of values at different levels (Table 2). The implication of this simulation result is that the influence of soil or root distribution on the total forest evapotranspiration should be conveyed through the effect on the function of overstory trees.
3.5. Can Capillary Rise Sustain Transpiration?
Figure 7illustrates the cumulative upward flux into the root zone of each tree type obtained for the entire simulation period. Only results for the shallower root scenarios are shown, as the fluxes were zero for all soil-root combinations in the case of scenarios corresponding to the maximum root depthZRootD. The total upward flux is very small for all considered cases and represents smaller than 100th of a percent of the total annual transpiration by trees at all levels. The results are consistent among all soil types. This result is also consistent with Markewitz et al. , who focused on soil moisture dynamics at the site of rainfall exclusion experiment [Nepstad et al., 2002].
 Note that the simulated subsurface water dynamics may exhibit minor diurnal fluctuations of near-surface soil moisture (not shown). They are related to the capillary effects of periodically developing high gradients of soil water potential during dry periods. Specifically, moisture decreases during daytime because of transpiration and slightly increases at nighttime because of the capillary pull of water from lower depths of higher wetness. These dynamics are more pronounced at shallow soil layers, similar to those shownda Rocha et al. [2004 Figure 3], dissipating with depth and becoming insignificant at the root zone bottom of any tree type represented by the model (e.g., Figure 7). Note that the simulated fluctuations are only related to the soil's effect; they have nothing to do with the effect of hydraulic redistribution (not implemented in the model). Yet, the latter mechanism was hypothesized by a number of studies to explain the empirical soil moisture data measured at the km 83 flux tower site of da Rocha et al. .
 Further, the relevant question one may ask is whether soil hydraulic properties exert a significant effect on the simulated dynamics of evapotranspiration and how they might be related to root vertical variability. One may conjecture, for example, that the “optimal” root distribution would minimize water losses from the root zone. The obtained results only partially corroborate such a statement (the results are only marginally sensitive with respect to the soil type: all used types result in nearly the same flux magnitudes). Specifically, the cumulative net flux from the root zone of overstory trees (obtained for the entire simulation period) exhibits a minimum for the root permutation scenarios 6–8 for ZRootD, and for the scenarios 8–11, in the case of ZRootS (see the auxiliary material, Figure S3). The losses from the root zones of understory trees and mid-canopy trees progressively increase for the deeper root scenarios; in the case of the shallower root scenarios, they reach a minimum for the scenarios 8–11. The modeled leakage is largest for understory and mid-canopy trees for the highest degree of root niche separation. This moisture could be considered a surplus for tall trees; however, one has to take into account the temporal dimension. Specifically, if only wet season leakage increases, there is no benefit for tall trees (they are light limited); if there is an increase in dry season leakage, this is indeed beneficial. Contrasting the notion that only deep roots are sufficient because they have access to a larger moisture reservoir, the moisture surplus can only occur when the uptake density for taller trees becomes smaller in the shallower layers. The corresponding positive effect on deep soil recharge is only obtained when root niche separation assumed.
4.1. Root Niche Separation as the Likeliest Expression of Adaptation Strategy
 Given the amount of water evaporated in the dry season, it is clear that roots must extend to several meters, and existing evidence indicates a depth range of at least 0–18 m [Davidson et al., 2011]. But one cannot unambiguously resolve the exact water-stress avoidance mechanism without additional direct information. This study further argues that existingindirectfacts point to the plausibility of the root-niche separation hypothesis. The remarkable agreement of outcomes of rainfall exclusion experiments described byNepstad et al. [2002, 2007] and da Costa et al. as well as a pan-tropical assessment of drought-related tree mortality byPhillips et al.  and observations for a neotropical forest system by Condit et al.  deserve particular consideration.
 Specifically, in the long-term rainfall exclusion experiment in Tapajós, detailed byNepstad et al. , a severe, 4-year drought episode was simulated by excluding 60% of throughfall during each wet season from a 1-ha forest treatment plot. After 3.2 years of the experiment, “surprisingly, the mortality of large trees began only during the final year of the experiment… the treatment resulted in … mortality rates increased 4.5-fold among large trees (> 30 cm dbh) and twofold among medium trees (10–30 cm dbh) … whereas the smallest stems were less responsive” [Nepstad et al., 2007]. The following discussion draws an important analogy between the outcomes of this particular field-monitoring program and inferences of this study.
 A priori, if a decrease of wet season precipitation is imposed in numerical simulations, a similar effect of vulnerability of overstory trees should emerge, provided the model realistically reproduces relevant processes of heat-water exchange. Obviously, the effect can only be expressed in terms of increased water stress, not mortality, as the latter cannot be reliably modeled in a deterministic fashion. In an attempt to verify this notion, a synthetic rainfall scenario was developed in which precipitation was reduced by 60% from January through June of each year, over the period of January 2002 to January 2006. The effort intended to mimic the design ofNepstad et al. , who carried out their monitoring in close proximity to the km 67 site but over a different period. Figure 8 illustrates a comparison of dynamics of the soil moisture availability factor obtained in simulations with observed and reduced wet season precipitation (see a similar illustration for a different soil type in the auxiliary material, Figure S4). The identical control root scenario and the scenario with distinct root niches (highest differences among the root profiles corresponding to trees at different canopy levels) were used. In simulations described in section 3.3, the control root scenario exhibited a maximum, while the scenario with distinct root niches exhibited minimum water stress, the stress being interpreted as . As seen in the figure, the latter scenario with observed (not decreased) precipitation forcing exhibits only minor water deficiencies during the three dry seasons, and they are only characteristic of understory trees. Overstory trees are not water limited, and this is the case for both soil types, as inferred from Figure 8.
 For the control root scenario, the 60% decrease of wet season precipitation leads to similar water stresses experienced by all trees (Figure 8, blue lines) because they have similar moisture conditions in their root profiles. Assuming that moisture sensitivity thresholds of all trees are the same, a similar physiological response to stress conditions would be an outcome. One can further conjecture that there must exist a correlation (albeit uncertain) between the heuristic factor and the probability of mortality, which would imply a nearly uniform mortality rates among all tree size classes than observed in the exclusion experiments. Given the uncertainty of such a correlation and that of mechanistic reasons of tree mortality [e.g., McDowell et al., 2008], the inference is nothing but a “first-order” expectation consistent with water stress hydraulic effects.
 For the scenario with distinct root niches, the 60% decrease of wet season precipitation does not affect water availability of understory and mid-canopy trees significantly: they are able to transpire most of the dry season and a fraction of wet season precipitation. Similarly, the effect of precipitation shortage is not appreciable for overstory trees until 2–2.5 years after the beginning of simulations. This implies that top-canopy trees exploit water surplus stored in deep soils recharged via earlier wet season rainfall. The water stress sets in toward the end of the second dry season and aggravates severely by the end of the fourth year. At that time, overstory trees cannot satisfy their water needs by more than 50% (Figure 8). Following the above argument of the existence of correlation between the factor and the probability of mortality, in actual conditions, one would expect an increase in mortality of overstory trees. To provide a better insight, Figure 9provides a graphical illustration of the soil water simulations, when the permutation scenario with distinct root niches is assumed. It shows the depletion of deeper soil moisture reservoir during the first 2–2.5 years. A subsequent aggravation of conditions for depths larger than 3.5 m is apparent, as wetting fronts resulting from rainfall events do not penetrate beyond that depth. The above results demonstrate that under the assumption of equal hydraulic efficiency, root niche separation is a plausible and consistent hypothesis that could explain both the observed robustness in forest productivity during dry seasons as well as vulnerability of canopy-dominant trees to sustained water loss during longer-term droughts.
4.2. What Are the Other Possible Alternative Hypotheses?
 Needless to say that this study has explored only a fraction of possible hypotheses that can be drawn for explaining what appears to be a robust drought avoidance strategy by the rainforest. Four alternative explanations closely related to what has been addressed in this study are qualitatively discussed below. Further research is needed to address them in full detail.
 Could simply the development of different rooting depths be a sufficient strategy? That is, overstory trees have deep roots, while shorter trees have shallower roots but the centroids of their uptake at potential rates overlap, i.e., taller trees do not exhibit a reduced density of moisture uptake in surface layers (for example, see the auxiliary material, Figure S5). Simulations carried out with such a characterizaton of root profiles lead to water stress that is even higher (see the auxiliary material, Figure S6) than that obtained when the control root scenario was used (Figure 8). This result underlines the importance of the uptake density distribution, with a conclusion that screening different depth ranges is insufficient; only deepening of the uptake centroid depth can result in transpiration approaching the potential rates.
 Can the “compensation” effect referred to in Appendix A make the above hypothesis of different rooting depths viable? In other words, are there intraseasonal adjustments of centroids of moisture uptake at potential rates or are they seasonally invariant (i.e., determined by the root biomass distribution, as was assumed in this study)?
 According to this hypothesis, all trees compete equally for moisture in shallow soil, but as this reservoir is becoming depleted, water uptake shifts deeper in the soil profile, partitioning their uptake throughout the root profile, depending on soil condition. Under this conceptualization, overstory trees with deeper roots would have a larger capacity for adjustments. Given how little is understood about the conditions under which the compensation effects are appropriate to consider and a feasible degree of such a “plasticity” [Couvreur et al., 2012; Doussan et al., 2006; Javaux et al., 2008; Schneider et al., 2010; van Lier et al., 2008], any speculation is quite uncertain. Without getting into a deeper, unsubstantiated discussion, the results of the rainfall exclusion experiment in Tapajós appear to be in qualitative disagreement with such a hypothesis. Specifically, if large trees were competing for shallow soil moisture equally with shorter trees, large trees would exploit shallow soil reservoir to the best they can, satisfying their high energy inputs. In conditions of the experimental drought, there would be little water left for understory trees within the reach of their shallower root zone. Pronounced signs of stress and an increased mortality would be expected for small trees, while larger trees would thrive. This is directly opposite to what was observed in the Tapajós Forest.
 The possibility of the hydraulic redistribution mechanism was previously addressed for this rainforest system from the perspective of a single lumped vegetation layer [Markewitz et al., 2010]. Recently, Quijano et al.  employed this mechanism to explore interactions between vegetation species stratified according to their positions within canopy and soil layers for a Mediterranean climate system. Mutualistic dependencies identified in the study of Quijano et al.  could possibly play out in the context of differences among rooting profiles hypothesized for the study site. However, direct conjectures are difficult, and further research is needed to determine the plausibility of facilitation of moisture resource use.
 Lastly, one cannot fully exclude a dependence of drought tolerance on tree size/age that was not considered in this study. “Vulnerability curves” [Sperry et al., 2002] of taller (and presumbly older) trees can differ from those of younger, shorter trees. Although theoretically feasible, clear evidence supporting such a statement is yet to be presented in research literature.
4.3. Does Root Niche Separation Make Evolutionary Sense?
 The biological mechanism of root niche separation is consistent with an adaptive response to the tradeoff between the requirement for sustained productivity for canopy-dominant trees and the risk of sustained soil water limitation from historically infrequent extended droughts in the moist tropics. While the hypothesis appears to be a robust explanation at the system scale, is it consistent with an emergence of evolutionary advantages for an individual?
 First, a large tree does need access to larger and buffered storages of soil water to survive droughts, so it needs deep roots. And, in theory, the increased deep root growth can represent an evolved physiological response to breaking into the canopy level (i.e., increase of exposure to light) and a concomitant increase in the aboveground biomass. In this regard, the empirically observed narrow range of root:shoot ratios is not violated. Second, the study argues that while becoming large, a tree needs to reduce its root water uptake in surface layers, where it competes for the same resource with smaller/understory trees. While at a first glance this could be interpreted as an expression of “altruism,” it is not so because plant locations are fixed and trees need to adapt not only to climatic environment but also to the “boundary conditions” created by other members of the community; in other words, smaller/understory trees, or, rather, their effects on soil water content, can be considered a part of physical environment of large trees. If such shifts can be indeed assumed, their existence is a response to a long-term condition of mutually unfavorable competition for moisture in surficial layers. Specifically, what emerges from this study is that by growing deeper roots and reducing surficial root uptake, a large tree positively affects the hydrological component of its environment: (1) the downward shift of uptake increases soil moisture in surface layers (2) that leads to a (very) nonlinear growth of soil hydraulic conductivity, and (3) therefore, recharge of deeper soil layers, (4) by reducing the density of uptake in surface layers, a tree compensates the atmospheric demand with a higher uptake at deeper locations. Note that the reduced surficial water uptake can be achieved through (1) a reduction of fine root biomass (i.e., “giving up” this biomass cannot affect the root:shoot ratio appreciably, as coarse roots constitute most of the belowground biomass) or (2) through a possible physiological adjustment of root conductivity in surface layers. Specifically, shallow roots with lower conductivity (which is reasonable to assume through a possibility of tissue suberization or other adaptations) would imply their relatively smaller role for the same biomass. Both of these scenarios were incorporated within the modeling framework indirectly through the functions of relative uptake density. By screening a larger depth and moving the centroid depth of uptake, a large tree effectively separates its uptake niche from that of a shorter tree. Over the evolutionary time, this could represent a stable strategy for trees to survive episodic (but recurrent) droughts, and individuals who failed to create such a separation would fail functionally and therefore evolutionarily.
 Lastly, from the perspective of “cost-benefit” analysis, allocation of carbon to deep roots can make evolutionary sense only if this results in a positive carbon increment over a long term. The ability to respond positively to enhanced light conditions during droughts, thereby achieving higher photosynthetic activity and increased CO2 uptake [e.g., Goulden et al., 2004; Huete et al., 2006; Hutyra et al., 2007; Myneni et al., 2007; Saleska et al., 2007, 2003; da Rocha et al., 2004], would appear to be such a benefit necessarily augmenting any water stress avoidance strategy. A robust increase of carbon assimilation during dry seasons at the study site (for details, see Hutyra et al. ) indicates that the two effects could indeed coexist, possibly implying the long-term advantage in terms of costs associated with growth and maintenance of deep roots.
4.4. Wet Season Rainfall as a Key Factor of Ecosystem Stability
 Qualitatively comparing Figure 9 with Figure 5, one may propose that by wetting the deep soil profile (down to 36 m, not shown), wet season precipitation has a resetting effect on soil water, so that plants transit to dry season conditions with plentiful moisture. Because overstory trees can access deeper soil layers, moisture that takes its origin from wet season rainfall may exert a buffering effect on severity of drought conditions of any particular year (or two, as the simulations demonstrate). However, if dry conditions persist for a period of time longer than the duration of the buffering effect, overstory trees will reach a “tipping” point in water stress, and a sharply growing mortality would be the outcome. Mid-canopy and understory trees will also likely become vulnerable to drought conditions once the overstory canopy becomes thinner, and a stronger drying effect is exerted on these trees. A further nonlinear effect would be a drier, combustible litter layer in the forest floor [Ray et al., 2005] that would enhance the occurrence of fires and further enhancement of tree mortality [Cochrane et al., 1999]. Consequently, one may conclude that interannual variability of wet season precipitation is an important characteristic of seasonality that affects stability of the rainforest system at the location of the study site. Currently, global climate models project an increase of dry-season intensity in the eastern and southestern Amazon, while as a reduction of wet-season rainfall appears to be less likely [Costa and Foley, 2000; Malhi et al., 2008, 2009]. Serious deficiencies in the structure and resolutions of climate models, however, suggest that the uncertainty of these projections could be very high [Davidson et al., 2012; Malhi et al., 2009]. By missing accurate representation of key atmospheric phenomena, most climate models tend to underestimate current rainfall and, in particular, wet-season precipitation. Relative seasonal changes of rainfall commonly used in climate impact analyses [Malhi et al., 2009] thus could exhibit “dampened” sensitivities.
 One may further hypothesize that long-term reduction of wet season precipitation can reach a threshold (see theauxiliary material, section S.6) sufficient to cause complete mortality of overstory trees, defined by inadequate amounts to recharge deep soils for 2–3 years in succession. Although the subsequent transient changes of the forest structure and differential responses of tree species are hard to assess, it is not impossible that the same threshold would represent a tipping point for the entire system, potentially leading to a collapse of the evergreen equatorial biome and its replacement by seasonal forest. This result agrees with the factors limiting the extent of evergreen forests inferred from the biogeographical study of Hutyra et al. . This study found that forests were extant (prior to deforestation) up to a boundary where the accessible soil depth became depleted on average for two or more successive years; i.e., a very different approach led to a similar answer to what was inferred here.
4.5. Robustness of Results
 The model uses a number of parameters that are reported in Tables 1 and 2. The ensemble of scenarios of soil hydraulic properties was assumed to include the possible variability of soil textures that occur in the vicinity of the flux tower site [Hutyra et al., 2007]. As the results demonstrate, the essential inferences of the study are not sensitive with respect to a soil hydraulic parameterization.
 The parameter space of several vegetation characteristics (section 3.1) has been explored during manual model calibration efforts. However, the parameter values were kept within their narrow “biophysically realistic” ranges that were accepted in numerous previous land-surface modeling studies [e.g.,Bonan, 2008]. This ensures consistency with field-measured values.
 The sensitivity of modeling results was further explored (see the auxiliary material, section S.7) with respect to two parameters that are particularly uncertain and hard to infer from in situ observations. They are the soil water potential at which stomatal closure begins, , and the soil water potential at which plant wilting begins, (Table 2). For lower and higher magnitudes of these parameters, as compared to the values used in all above simulations, the inferences of the study remain the same, as detailed in the auxiliary material, section S.7.
4.6. Potential Applicability to Other Rainforest Areas
 One may further investigate the applicability of the root niche separation hypothesis to other sites, where higher mortality of canopy-dominant trees has been observed following drought episodes. For example,Phillips et al. report that large trees are most vulnerable to drought-related mortality, based on an analysis of the response in forest plots to a short, intense drought in the Amazon in 2005. Yet, it is hard to reconcile what appears to be a fairly high sensitivity of tree mortality reported byPhillips et al. study (e.g., increased mortality following a single intense drought) with an apparently more robust forest system in the Tapajós rainfall exclusion experiment, where increased mortality was not observed until the third year, which is about the same time lag necessary for reaching the drought-related tipping point in this modeling study. It may be difficult to draw analogies because the results ofPhillips et al. do not allow the evaluation of the circumstances under which mortality occurred at different sites. Such circumstances could include (1) the dynamics of pre-2005 rainfall (i.e., consistently low annual totals could have initiated drought signal buildup in deep soil before the severe 2005 episode; e.g.,Brando et al. reported a decrease of wet-season precipitation and an increase of radiation flux for the Amazon Basin over the period of 1996–2005), (2) the increased temperatures and radiation load caused by the real drought (absent from the experimental manipulation), (3) the age characteristics of dying trees (e.g., older trees might be more vulnerable because of imperfections in xylem developed over time), and (4) the differential effects of soil textures. Overall,Phillips et al. data neither strongly confirm nor contradict the hypothesis of root niche separation. More specific, site-level analysis could help identify whether the observation is in agreement/disagreement with the proposed hypothesis.
 The main outcomes of the other long-term rainfall exclusion experiment in the Caxiuanã National Forest in the eastern Amazon [da Costa et al., 2010] were very similar to the results of the experiment in Tapajós. Several hydrologically significant differences may be noted for that site: (1) shallower, 10–15 m deep, sandy Oxisol soils; (2) periodic wetting by groundwater at 10–15 m during wet seasons, which most certainly limits the maximum rooting depth, and (3) rainfall was excluded both during dry and wet seasons (although da Costa et al. note that dry seasons in Caxiuanã exhibit higher precipitation than in Tapajós). One may therefore suggest that in the Caxiuanã National Forest: (1) because of a more conductive soil, rainfall more rapidly recharges deeper layers, so large trees may also benefit from dry season rainfall; (2) overstory trees have the opportunity to pull shallower groundwater by capillarity (water table is not affected by rainfall exclusion because of flow advection in the saturated zone), in the case a drought signal develops in soil; and (3) understory-medium trees had somewhat less advantage, as compared to the conditions in Tapajós, because of both experimentally reduced dry season rainfall and more conductive soil. This is consistent with the observation that drought-induced mortality in the Caxiuanã experiment was not as strongly skewed toward large trees as it was in the Tapajós experiment. All of these arguments are consistent with the proposed hypothesis of root niche separation. A more detailed analysis could provide more insight and a stronger possible confirmation, but this is beyond the scope of the current study.
 This study has addressed linkages between the subsurface moisture dynamics and evapotranspiration of a mature Amazonian rainforest. As in any modeling study, a number of simplifying assumptions were made, which are particularly difficult to avoid for such a complex system as the rainforest. Despite the fairly simple structure, the model mimics essential processes of heat flow and storage with a particular emphasis on soil hydraulics and the effects of tree root distribution on soil moisture dynamics. Specific model assumptions and parameterizations are detailed in the earlier work of Ivanov et al. , the auxiliary material (sections S.3 and S.4), and Appendix A.
 The study clearly indicates that the soil's capillary action, i.e., the upward unsaturated water flow caused by the gradient in soil matric potential due to transpirational uptake, is not a sufficient mechanism to explain stress avoidance. The conclusion is consistent for all soil types used in the study.
 The results indicate that rainforest trees can have at least two or, most likely, a combination thereof, drought avoidance strategies at the study site. First, all trees may have a very deeproot structure with the majority of roots located in the top soil layer and progressively smaller densities at deeper locations. The open question remains though whether such deep rooting is feasible in terms of plant-soil hydraulics and soil diffusion of oxygen required for root functioning. This study has not addressed this question and detailed/mechanistic models of tree hydraulics and soil processes of gas diffusion would be needed.
 The second proposed strategy is that roots of trees located at different canopy levels occupy particular water uptake niches in the soil profile. Specifically, overstory trees receive most radiation and transpire the largest amounts of water; to reduce the risk of vulnerability due to seasonally fluctuating rainfall, these trees developed root systems that are more uniform and extend to larger depths to exploit deeper soil moisture. During drought episodes, fractionally higher root biomass at deeper soil locations (as compared, for example, to the bulk root profile shown in Figure 2a) permits access to moisture originating from wet season precipitation. In contrast, because understory trees are constantly light limited, their relative contribution to the total transpiration flux is significantly smaller as compared to the contribution of overstory trees. They therefore do not need to allocate roots deeply, and their root profiles contain a majority of biomass in shallow surface layers. Full access to water from infrequent dry season precipitation events (and, possibly, a limited access to wet season moisture during drought periods) is sufficient for these trees to avoid seasonal drought stress. Evidence of mortality of large overstory trees by Nepstad et al. at a nearby location in Tapajós and conceptually the same inference from a numerical experiment of reduction of wet season rainfall demonstrated in this study (i.e., the lagged response to drought) confirm that root niche separation is a plausible and consistent expression of the mechanism of drought avoidance strategy at the study location. Furthermore, a remarkable consistency of postdrought mortality of large, canopy-dominant trees across a number of sites including Tapajós, the location of another rainfall exclusion experiment in the eastern Amazon [da Costa et al., 2010], and other tropical areas [Phillips et al., 2010] indicates that differential rooting depth may represent a more geographically widespread mechanism of adaptation of tropical trees to seasonal and interannual drought episodes. The previous biogeographical study of Hutyra et al. also supports the view that recharge of the deep soil reservoir every 2 years, or more often, is sufficient to support evergreen Amazonian forests, but forests do not survive in areas with less-frequent wetting of deep soils.
 Whether the hypothesized mechanism is an accurate representation of forest function can be tested with future observations, either of functional response to removal/girdling of large trees or of outcomes of further experiments with rainfall manipulation, or directly, of the size dependence of tree root profiles. For example, if root distributions are independent of tree size, large tree removal should cause a reduction, but no change in the distribution, of soil water removal by roots. The root niche separation hypothesis, by contrast, predicts that large tree removal would cause soil water removal profiles to shift upward. This latter prediction was recently confirmed by observations following selective removal of large trees [Miller et al., 2011] at the km 83 site; this study found a 40% decrease in water withdrawal in the logging gap relative to the intact area and a shift of water withdrawal to shallower locations. Direct observations of size dependence of intact forest tree-root profiles have not previously been possible, but new “barcoding” methods based on matching DNA sequences in belowground roots to aboveground components suggests that direct observational tests may soon become feasible [Jones et al., 2011].
 Overall, the proposed biological mechanism of root niche separation is in a conceptual accordance with an adaptive response to the tradeoff between the evolutionary requirement of sustained productivity and the risks imposed by soil water limitation. By mining deep soil moisture, overstory trees exploit the buffering effect of wet season precipitation against severity of drought conditions of any particular year. As explored here in a number of synthetic scenarios, wet season rainfall therefore represents a vital characteristic affecting the stability of the rainforest system at the location of the study site.
Appendix A:: Soil Moisture Availability Factor
 A heuristic soil moisture availability factor (−) is used to regulate the stomatal conductance based on the soil moisture distribution in the root zone. The formulation is based on a widely used relationship [Bonan, 1996; Feddes et al., 2001]:
where index refers to a depth zj (m) (zero at the ground surface, positive downward) of the soil profile mesh; the root biomass fraction (−) is estimated such that ; is the volumetric soil moisture content; is the moisture content corresponding to the water potential at which plant wilting begins (Table 2); and is the threshold moisture content corresponding to the water potential at which stomatal closure begins (Table 2). Note that and depend on the selected soil water retention parameters (Table 1), following the model of van Genuchten .
 From equation (A1), the lumped factor explicitly accounts for soil moisture variability within the root profile by using appropriate weights of root biomass . Note that the above approach with root-specific densities used as weights to quantify water stress effect is a simplified representation of the uptake function. Plants have been observed to change their active root water uptake zones to meet transpiration needs even when some parts of the root system are water stressed [e.g.,Garrigues et al., 2006; Sharp and Davies, 1985]. When shallow soil layers (typically containing higher root densities) dry out, plants seem to be capable of shifting water uptake to deeper and moister soil layers to compensate for the decreased root water uptake from shallower layers [Doussan et al., 2006; Javaux et al., 2008; Schneider et al., 2010]. Apparently, such a “compensation” effect cannot be modeled by using the “Feddes-type” approach presented above, and this can be considered a model limitation. However, the degree to which such compensation effects can be expressed is still not well understood, and further research is needed to provide more mechanistic alternatives to the common parameterizationequation (A1).
 The departure of from unity indicates soil control on the transpiration flux and water limitation; can thus be interpreted as a metric of water stress experienced by vegetation. Specifically, factors are computed at each time step of the energy partition model (hourly) for each individual tree type. The resultant lumped factor is then used to constrain the maximum catalytic capacity of Rubisco Vmax [Ivanov et al., 2008, equation (B9)], which in nonlimiting light conditions affects photosynthesis and therefore the stomatal resistance of the plant. Note that the stomatal resistances are computed separately for sunlit (“sun”) and shaded (“shd”) fractions of each of the canopy layers, and (see Ivanov et al. [2008, Appendix B] for details). In total, six dynamic canopy fractions are thus represented in this model implementation. Upscaling of sunlit and shaded fractions to the level of each individual canopy layer is subsequently carried out (auxiliary material, equation (S-6)).
 Through their effect on the stomatal resistance, the factors affect the estimation of latent heat flux . Subsequently, factors are used as weights for specifying the moisture sinks associated with transpiration in the model of soil water dynamics. Note that depends on the soil hydraulic parameterization, the root profile, and the soil water distribution with depth.
 This work was supported by Ziff Postdoctoral Fellowship at the Center for the Environment at Harvard University and partially by the NSF grant 0911444. The technical support of the Center for Advanced Computing at the University of Michigan is acknowledged. Help of Simone Fatichi with data processing is highly appreciated. Insightful comments from three anonymous reviewers have led to substantial improvements of the manuscript material presentation.