Study of the Th1/Th2 balance, including IL-10 production,in cultures of peripheral blood mononuclear cells frombirch-pollen-allergic patients
Article first published online: 24 DEC 2001
Volume 55, Issue 2, pages 171–175, February 2000
How to Cite
Movérare, R., Elfman, L., Stålenheim, G., Björnsson, E. and Movérare, R. (2000), Study of the Th1/Th2 balance, including IL-10 production,in cultures of peripheral blood mononuclear cells frombirch-pollen-allergic patients. Allergy, 55: 171–175. doi: 10.1034/j.1398-9995.2000.00244.x
- Issue published online: 24 DEC 2001
- Article first published online: 24 DEC 2001
- Accepted for publication 26 August 1999
- birch pollen;
Background: Excessive production of interleukin (IL)-4, IL-5, IL-10, and IL-13 is thought to be important in the development of allergy and asthma. The objective of this investigation was to study Th1/Th2-like cytokine profiles in vitro in seven patients allergic to birch pollen and six nonallergic controls during the birch-pollen season.
Methods: Peripheral blood mononuclear cells (PBMC) were isolated and cultured with birch-pollen extract (BPE) or tetanus toxoid (TT) for 7 days, harvested, and restimulated with the mitogens phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) for 24 h. Cytokine production was determined by ELISA, and logarithmic cytokine ratios were compared between the two groups and between the antigens.
Results: In the allergic group, the cultures prestimulated with BPE had a more Th2-like cytokine response than the TT-prestimulated cultures; i.e., lower IFN-γ and higher IL-10 production (P<0.05), as well as higher IL-5/IFN-γ and IL-13/IFN-γ ratios (P<0.05). There were also significantly higher IL-4/IFN-γ (P<0.005) and IL-5/IFN-γ (P<0.05) ratios in BPE-stimulated cultures in the allergic group than in the control group. The IL-4 and IL-13 production in vitro correlated with the specific serum IgE levels.
Conclusions: BPE stimulation induces a Th2-like cytokine response by PBMC isolated during the pollen season from birch-pollen-allergic patients, indicating a Th2-type immune response to birch pollen in vivo.