Molecular features determining lymphocyte reactivity in allergic contact dermatitis to chloramphenicol and azidamphenicol
Article first published online: 23 SEP 2008
Volume 56, Issue 1, pages 69–72, January 2001
How to Cite
Sachs, B., Erdmann, S., Al Masaoudi, T. and Merk, H. F. (2001), Molecular features determining lymphocyte reactivity in allergic contact dermatitis to chloramphenicol and azidamphenicol. Allergy, 56: 69–72. doi: 10.1034/j.1398-9995.2001.00701.x
- Issue published online: 23 SEP 2008
- Article first published online: 23 SEP 2008
- Accepted for publication 14 July 2000
- contact dermatitis;
- lymphocyte transformation test;
- patch test
Background: We report on two cases of allergic contact dermatitis to chloramphenicol and azidamphenicol respectively, with in vivo and in vitro lymphocyte reactivity to both compounds. The molecular features determining lymphocyte reactivity were explored because chloramphenicol, azidamphenicol, and thiamphenicol exhibit almost identical chemical structures.
Methods: With chloramphenicol, azidamphenicol, and the chemically related thiamphenicol, we performed patch tests and lymphocyte transformation tests with both patients. Furthermore, the interleukin-5 and interferon-γ concentrations in the cultures of peripheral blood mononuclear cells of one patient were determined.
Results: Patch tests showed delayed hypersensitivity reactions to chloramphenicol and azidamphenicol, but not to thiamphenicol. These results were confirmed by lymphocyte transformation tests with peripheral blood mononuclear cells of the patients, showing a proliferative T-cell response to azidamphenicol and chloramphenicol. Moreover, lymphocytes from one patient secreted large amounts of interleukin-5, but not of interferon-γ upon coculture with azidamphenicol.
Conclusions: Since lymphocyte reactivity was observed to chloramphenicol and azidamphenicol, but not to thiamphenicol, the epitope(s) recognized by the allergen-reactive T cells may be formed by the nitro-group of the benzene ring shared by chloramphenicol and azidamphenicol.