Cytokine production from sputum cells after allergenic challenge in IgE-mediated asthma
Version of Record online: 11 DEC 2002
Volume 57, Issue 12, pages 1145–1150, December 2002
How to Cite
Bettiol, J., Sele, J., Henket, M., Louis, E., Malaise, M., Bartsch, P. and Louis, R. (2002), Cytokine production from sputum cells after allergenic challenge in IgE-mediated asthma. Allergy, 57: 1145–1150. doi: 10.1034/j.1398-9995.2002.23586.x
- Issue online: 11 DEC 2002
- Version of Record online: 11 DEC 2002
- Accepted for publication 8 July 2002
- cell culture;
Background: Th2 cytokine production from airway cells is thought to govern the eosinophilic airways inflammation in allergic asthma. Induced sputum has become a widely used technique to assess airways inflammation.
Methods: By applying the technique of induced sputum to collect airways cells, we have assessed the spontaneous production of a set of cytokines, including interleukin-4, 6, 10, interferon-γ and tumour necrosis factor-α, 6 h after a bronchial allergenic challenge with Dermatophagoides pteronyssinus (Dpt) in 12 sensitized asthmatics and compared the results obtained after inhalation of saline as control. A group of eight healthy non-allergic subjects was enrolled to control for any non-specific effect of Dpt. Cytokines were measured by a dynamic immunoassay during a 24-h sputum cell culture.
Results: Allergen challenge in sensitized asthmatics caused an acute and a late bronchospasm together with a rise in sputum eosinophil counts. Afterwards allergen sputum cells from allergic asthmatics displayed a rise in their production of IL-4 (P < 0.01), IL-6 (P < 0.05) and IL-10 (P < 0.05) when compared to saline. By this time sputum generation of IL-4 in atopic asthmatics was greater than in healthy subjects (P < 0.001). Furthermore, in allergic asthmatics there was a strong correlation between the rise in interleukin-4 production from sputum cells and the rise in sputum eosinophils (r = 0.87, P < 0.001).
Conclusions: Sputum cell culture is a useful model to assess cytokine production in allergic asthmatics who show a marked up-regulation of Th2 cytokines following acute allergen exposure. The rise in sputum eosinophil count following allergen challenge strongly correlates with the rise in IL-4 generation from sputum cells.