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Molecular analysis of HLA-DRB1, -DQA1 and -DQB1 polymorphism in Turkey

Authors


Correspondence to:Dr. Güher Saruhan-Direskeneli
İstanbul Üniversitesi İstanbul Tıp Fakültesi
Fizyoloji AD 34390
Çapa Istanbul
Turkey
Tel/Fax: +90 212 533 94 68
e-mail: gsaruhan@istanbul.edu.tr

Abstract

Acknowledgments:

This study is supported by a grant (SBAG1529) from TÜBITAK (Turkish Research Council) and the Research Fund of the University of Istanbul.

Abstract:

We report the evaluation of MHC class II polymorphism in the population of Turkey. HLA-DRB1, -DQA1 and -DQB1 have been investigated by polymerase chain reaction and sequence-specific oligonucleotide probe hybridisations (PCR/SSO) and sequence-specific priming (SSP) in 250 randomly selected healthy individuals. We also report the allelic distribution of these genes. The most frequent alleles detected were DRB1*1101 (0.104), *0301 (0.092), *0701 (0.090), DQA1*0501 (0.334), *0102 (0.164) and *03 (0.148) and DQB1*0301 (0.256), *02 (0.164), *0302 (0.128). The frequent ‘putative’ three-locus haplotypes carry the most frequent alleles at these loci. The most frequently detected class II “haplotypes” are DRB1*1101 DQA1*0501 DQB1*0301 (0.100), DRB1*0301 DQA1*0501 DQB1*02 (0.092) and DRB1*0701 DQA1*0201 DQB1*02 (0.072). The distribution of alleles and ‘putative’ haplotypes has shown common features with other Mediterranean populations. The results extend the HLA map to another Mediterranean country and provide a database for further HLA-disease association studies and transplantation applications.

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