Complete cDNA sequences of the HLA-DRB1*14011, *1402, *1403 and *1404 alleles

Authors


  • The complete cDNA sequences of the HLA-DRB1*14011, *1402, *1403 and *1404 alleles have been deposited in the EMBL data library and have the following accession numbers: AJ297582 (HLA-DRB1*14011), AJ297583 (HLA-DRB1*1402), AJ297584 (HLA-DRB1*1403) and AJ297585 (HLA-DRB1*1404). The names DRB1*14011, *1402, *1403 and *1404 have been officially assigned by the WHO Nomenclature Committee for Factors of the HLA System in August 1989 (the two former) and November 1990 (the two last). This follows the agreed policy that, subject to the conditions stated in the most nomenclature report (1), names will be assigned to new sequences as they are identified. All sequences have been submitted to the IMGT/HLA Sequence database (2).

Dr. Steven G.E. Marsh
Anthony Nolan Research Institute
The Royal Free Hospital
Pond Street, Hampstead
London NW3 2QG
UK
Tel: +44 (0) 20 7284 8321
Fax: +44 (0) 20 7284 8331
email: marsh@ebi.ac.uk

Abstract

Abstract: Sequencing studies of HLA class II molecules have been focused almost exclusively on the highly polymorphic exon 2. In this study the complete cDNA sequence of four alleles of the DR14 lineage (DR52 group) are reported for the first time. The HLA-DRB1*1402 and *1403 sequences were shown to be identical to the previously determined DRB1*13011 sequence, also of the DR52 group, in exons 1, 3, 4, 5 and 6. HLA-DRB1*14011 and *1404 were identical to DRB1*13011 in exons 1, 4, 5 and 6 sequences while they showed specific features within their exon 3 sequence. Both alleles showed a synonymous substitution at the third base of codon 114. However, DRB1*14011 also has a non-synonymous substitution at the first base of codon 112 which results in a histidine to tyrosine substitution. This is a novel substitution as Histidine 112 is conserved in all known HLA class II B genes.

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