Supported by GlaxoWelcome Australia, The Alfred Hospital Foundation and the National Health and Medical Research Council of Australia.
Modulatory effects of alveolar macrophages on CD4+ T-cell IL-5 responses correlate with IL-1β, IL-6, and IL-12 production
Article first published online: 25 DEC 2001
European Respiratory Journal
Volume 14, Issue 1, pages 106–112, July 1999
How to Cite
Tang, C. , Rolland, J.m., Ward, C. , Li, X. , Bish, R. , Thien, F. and Walters, E.H. (1999), Modulatory effects of alveolar macrophages on CD4+ T-cell IL-5 responses correlate with IL-1β, IL-6, and IL-12 production. European Respiratory Journal, 14: 106–112. doi: 10.1034/j.1399-3003.1999.14a18.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
Increasing evidence suggests that the pattern of T-cell cytokine production can be modulated by antigen presenting cell (APC)-derived factors during the cell interactions. Recently, it has been shown that alveolar macrophages (AMs) from atopic asthmatics (AA) but not atopic nonasthmatics (AN) enhance interleukin (IL)-5 production by CD4+ T-cells.
The present study compared AM production of IL-1β, IL-6, and IL-12, as well as their associated functional capacity to influence IL-5 production by allergen-specific CD4+ T-cells in 10 AA, 10 AN, and nine nonatopic control subjects (C).
AMs from AA showed a relatively high production of IL-1β and IL-6 (p<0.05) and a relatively low secretion of IL-12 compared to C, whereas AMs from AN and C behaved similarly. This study confirmed previous findings that co-culture with AMs augments IL-5 production from allergen-stimulated CD4+ T-cells only in AA and not in nonasthmatics even if they are atopic. On the other hand, stimulation with allergen alone did not enhance IL-5 production by CD4+ T-cells in either AA nor AN. AM-induced changes in CD4+ T-cell IL-5 production upon allergen stimulation significantly correlated with their ability to produce IL-1β (r=0.59, p<0.01), IL-6 (r=0.56, p<0.01), and inversely with IL-12 (r= -64, p=0.002) in all atopic subjects, and even more closely with the ratio of IL-12/IL-1β (r= -0.75, p<0.001) and IL-12/IL-6 production (r= -0.81, p<0.001) in these subjects.
These findings suggest that the role of alveolar macrophages from atopic asthmatics in enhancing interleukin-5 production by allergen-specific CD4+ T-cells is due, at least partly, to their aberrant production of interleukin-1β, interleukin-6, and particularly of interleukin-12.