This study has been supported by the Biomed 2 program from the European Commission. Grant No: BMH4-CT96-0105.
Cytokine release from the nasal mucosa and whole blood after experimental exposures to organic dusts
Version of Record online: 25 DEC 2001
European Respiratory Journal
Volume 16, Issue 1, pages 140–145, July 2000
How to Cite
Sigsgaard, T., Bonefeld-Jørgensen, E.C. , Kjærgaard, S.k., Mamas, S. and Pedersen, O.f. (2000), Cytokine release from the nasal mucosa and whole blood after experimental exposures to organic dusts. European Respiratory Journal, 16: 140–145. doi: 10.1034/j.1399-3003.2000.16a25.x
- Issue online: 25 DEC 2001
- Version of Record online: 25 DEC 2001
- Cited By
- β-1,3- d-glucan ;
- cytokine release;
- individual susceptibility;
- nasal challenge;
- recycling workers;
- whole blood assay
The aim of this study was to assess the cytokine response after nasal exposure to organic dusts.
In a double blinded, crossover study five garbage workers with occupational airway symptoms and five healthy garbage workers were intranasally exposed to endotoxin (lipopolysaccharide LPS), β-1,3- d-glucan (GLU), Aspergillus sp., compost or the saline dilute for 15 min. Nasal cavity volume and nasal lavage (NAL) were performed at baseline and 3, 6, 11 h postexposure. NAL was analysed with differential cell counts, cysteinyl-leukotrienes, tumour necrosis factor α, interleukin (IL)-1β, IL-6 and IL-8. A whole blood assay on cytokine-release was performed with LPS and GLU.
NAL cytokines neutrophils, lymphocytes and albumin increased significantly at 6 h after LPS exposure. GLU induced an increase in albumin and a slight increase in IL-1β 6–11 h post exposure. In the WBA a significant increase in all cytokines after exposure to LPS as well as GLU was found. Significantly more cells were seen in NAL of the control group 6 h post LPS exposure.
In conclusion lipopolysaccharide is the most potent inducer of inflammation in the nasal mucosa whereas compost and β-1,3- d-glucan only induce minor changes. This reaction to lipopolysaccharide is attenuated in workers with occupational airway symptoms. In whole blood assay, however, β-1,3- d-glucan also induces cytokine release, indicating a different protective effect of the nasal mucosa towards lipopolysaccharide and β-1,3- d-glucan.