Cloning and expression of α-d-glucosidase and N-acetyl-β-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus)

  1. C. V. Hughes1,
  2. G. Malki1,
  3. C. Y. Loo1,
  4. A. C. R. Tanner2,
  5. N. Ganeshkumar1

Article first published online: 21 AUG 2003

DOI: 10.1034/j.1399-302X.2003.00091.x

Issue

Oral Microbiology and Immunology

Oral Microbiology and Immunology

Volume 18, Issue 5, pages 309–312, October 2003

Additional Information

How to Cite

Hughes, C. V., Malki, G., Loo, C. Y., Tanner, A. C. R. and Ganeshkumar, N. (2003), Cloning and expression of α-d-glucosidase and N-acetyl-β-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus). Oral Microbiology and Immunology, 18: 309–312. doi: 10.1034/j.1399-302X.2003.00091.x

Author Information

  1. 1

    Department of Pediatric Dentistry, Goldman School of Dental Medicine, Boston University, Boston, MA, USA, and

  2. 2

    The Forsyth Institute, Boston, MA, USA

*Dr N. Ganeshkumar, Department of Pediatric Dentistry, Goldman School of Dental Medicine, Boston University, 801 Albany Street, S-215, Boston, MA 02118, USA Tel.: +1 617 638 4773; fax: +1 617 638 5033 e-mail: nganesh@bu.edu

Publication History

  1. Issue published online: 21 AUG 2003
  2. Article first published online: 21 AUG 2003
  3. Accepted for publication April 21, 2003

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Keywords:

  • Tannerella forsythensis;
  • Bacteroides forsythus;
  • glycosidases;
  • glucosidase;
  • glycosaminidase

Screening a genomic library of Tannerella forsythensis (Bacteroides forsythus), using synthetic substrates conjugated to a fluorogen, 4-methylumbelliferone identified two glycosidase genes, which encode α-d-glucosidase and N-acetyl-β-d-glucosaminidase, respectively. The α-d-glucosidase has a Mr of 81,141 and is homologous to an α-d-glucosidase from Bacteroidesthetaiotaomicron. The N-acetyl-β-d-glucosaminidase has a Mr of 87,787 and is homologous to an N-acetyl-β-d-glucosaminidase in Porphyromonasgingivalis W83.

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