This paper is part of the contributions to the 1993 Annual Symposium of the Southern Section of the American Society of Plant Physiologists, held in Auburn, AL, USA, 20–22 March, 1993.
Genetic analysis of chloroplast biogenesis in higher plants
Article first published online: 9 OCT 2008
Volume 93, Issue 1, pages 163–170, January 1995
How to Cite
Barkan, A., Voelker, R., Mendel-Hartvig, J., Johnson, D. and Walker, M. (1995), Genetic analysis of chloroplast biogenesis in higher plants. Physiologia Plantarum, 93: 163–170. doi: 10.1034/j.1399-3054.1995.930123.x
- Issue published online: 9 OCT 2008
- Article first published online: 9 OCT 2008
- Received 24 June, 1994
- Cited By
- mRNA processing;
- nonphotosynthetic mutants;
The biogenesis of chloroplasts is genetically complex, involving hundreds of genes distributed between the nucleus and organelle. In higher plants, developmental parameters confer an added layer of complexity upon the genetic control of chloroplast biogenesis: the properties of plastids differ dramatically between different cell types. While the biochemistry and structure of different plastid types have been described in detail, factors that determine the timing and localization of chloroplast development and that mediate chloroplast assembly have remained elusive. To identify nuclear genes that play novel roles in chloroplast biogenesis, we are exploiting nuclear mutations that block the accumulation of subsets of chloroplast proteins. Detailed study of the mutant phenotypes provides clues concerning the primary defect in each mutant. Mutants with defects in chloroplast translation and mRNA metabolism have been identified. Other mutants defective in the accumulation of multiple thylakoid complexes show no apparent defect in the synthesis of the missing proteins. These may identify factors involved in the integration of proteins into the thylakoid membrane and their assembly into functional complexes.