Meta-topolin, an alternative to benzyladenine in tissue culture?

Authors

  • Stefaan P. O. Werbrouck,

    1. S. P. O. Werbrouck (corresponding author, e-mail stefaan.werbrouck@rug.ac.be) and P. C. Debergh, Dept of Plant Production - Horticulture, Univ. Gent, Coupure links 653, B-9000 Gent, Belgium; M. Strnad, Inst. of Experimental Botany, 77200 Olomouc, Czech Republic; H. A. Van Onckelen, Dept of Biology, Univ. of Antwerp, Belgium.
    Search for more papers by this author
  • Miroslav Strnad,

    1. S. P. O. Werbrouck (corresponding author, e-mail stefaan.werbrouck@rug.ac.be) and P. C. Debergh, Dept of Plant Production - Horticulture, Univ. Gent, Coupure links 653, B-9000 Gent, Belgium; M. Strnad, Inst. of Experimental Botany, 77200 Olomouc, Czech Republic; H. A. Van Onckelen, Dept of Biology, Univ. of Antwerp, Belgium.
    Search for more papers by this author
  • Henry A. Van Onckelen,

    1. S. P. O. Werbrouck (corresponding author, e-mail stefaan.werbrouck@rug.ac.be) and P. C. Debergh, Dept of Plant Production - Horticulture, Univ. Gent, Coupure links 653, B-9000 Gent, Belgium; M. Strnad, Inst. of Experimental Botany, 77200 Olomouc, Czech Republic; H. A. Van Onckelen, Dept of Biology, Univ. of Antwerp, Belgium.
    Search for more papers by this author
  • Pierre C. Debergh

    1. S. P. O. Werbrouck (corresponding author, e-mail stefaan.werbrouck@rug.ac.be) and P. C. Debergh, Dept of Plant Production - Horticulture, Univ. Gent, Coupure links 653, B-9000 Gent, Belgium; M. Strnad, Inst. of Experimental Botany, 77200 Olomouc, Czech Republic; H. A. Van Onckelen, Dept of Biology, Univ. of Antwerp, Belgium.
    Search for more papers by this author

Abstract

The metabolism and the in vitro effects of the cytokinin N6-(3-hydroxybenzyl)adenine (meta-topolin, mT) were compared with those of N6-benzyladenine (BA), N6-benzyl-9-(2-tetrahydropyranyl)adenine (BPA) and N6-benzyladenine-9-riboside ([9R]BA) in micropropagated Spathiphyllum floribundum Schott cv. Petite. In vitro, BA, BPA and [9R]BA were mainly converted into the stable derivative N6-benzyladenine-9-glucoside ([9G]BA), located at the basal part of the plant. N6-(3-O-β-D-glucopyranosyl)benzyladenine-9-riboside {(OG)[9R]mT} was the main derivative of mT. This new cytokinin-O-glucoside, which was present in all plant parts, was metabolized much faster than [9G]BA during acclimatization. The effect of BA and mT on in vitro shoot and root production and post vitro rooting was compared. Only mT combined a good shoot production with in vitro root formation. The plants that developed on medium with 10 µM or more mT rooted better during acclimatization than those developed on medium with equimolar concentrations of BA.

Ancillary