Beech trees exposed to high CO2 and to simulated summer ozone levels: Effects on photosynthesis, chloroplast components and leaf enzyme activity

Authors

  • Cornelius Lütz,

    1. Institute of Botany, University of Innsbruck, Sternwartestr. 15, A-6020 Innsbruck, Austria
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  • Sabine Anegg,

    1. Institute of Biochemical Plant Pathology, GSF – National Research Centre for Environment and Health, D-85764 Oberschleißheim, Germany
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  • Dominique Gerant,

    1. Laboratoire Biologie Forestière, Equipe Ecophysiologie Cellulaire, Université Henri Poincaré Nancy I, F-54506 Vandoeuvre Cedex, France
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  • Badr Alaoui-Sossé,

    1. Laboratoire Biologie Forestière, Equipe Ecophysiologie Cellulaire, Université Henri Poincaré Nancy I, F-54506 Vandoeuvre Cedex, France
    2. Laboratoire de Biologie et Ecophysiologie, F-25030 Besançon Cedex, France
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  • Joëlle Gérard,

    1. Laboratoire Biologie Forestière, Equipe Ecophysiologie Cellulaire, Université Henri Poincaré Nancy I, F-54506 Vandoeuvre Cedex, France
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  • Pierre Dizengremel

    1. Laboratoire Biologie Forestière, Equipe Ecophysiologie Cellulaire, Université Henri Poincaré Nancy I, F-54506 Vandoeuvre Cedex, France
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Corresponding author, e-mail: cornelius.luetz@uibk.ac.at

Abstract

Young trees of European beech (Fagus sylvatica L.) were exposed in a phytotron to different levels of ozone and CO2 under the climatic simulation of one vegetation period. High ozone levels were simulated similar to high ozone concentration in the field (up to 110 ppb), while CO2 was added as 300 ppm to the present level of ca 380 ppm. Our study describes different aspects of photosynthesis from the leaf level to the reactions of selected thylakoid components at different harvest times during growth of the beech trees under the different fumigation regimes. Ozone effects appeared in the first weeks of the treatment as a stimulation of chlorophyll fluorescence (Fv/Fm), in oxygen production and in ribulose-1,5-bisphosphate carboxylase/oxygenase activity, while the summer and early autumn harvests showed strong reductions in these parameters. Only phosphoenolpyruvate carboxylase (PEPcase) activity remained higher under high ozone. The effects of high CO2 appeared in general as a small stimulation in enzyme activity like PEPcase in spring. However, with increasing time of fumigation, reductions of all parameters were observed. Especially chlorophylls showed strong reductions under high CO2. The combined treatment with high ozone plus high CO2 resulted mostly in an amelioration of the negative ozone effects, although control levels were not reached.

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