Get access

Reduced inositol content in lymphocyte-derived cell lines from bipolar patients

Authors

  • RH Belmaker,

    1. Stanley Foundation Research Center Beersheva, Israel,
    2. Ministry of Health Mental Health Center Beersheva, Israel,
    3. Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel,
    Search for more papers by this author
  • J Shapiro,

    1. Stanley Foundation Research Center Beersheva, Israel,
    2. Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel,
    Search for more papers by this author
  • E Vainer,

    1. Stanley Foundation Research Center Beersheva, Israel,
    2. Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel,
    Search for more papers by this author
  • L Nemanov,

    1. Herzog Hospital, Jerusalem, Israel,
    Search for more papers by this author
  • RP Ebstein,

    1. Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel,
    2. Herzog Hospital, Jerusalem, Israel,
    Search for more papers by this author
  • G Agam

    1. Stanley Foundation Research Center Beersheva, Israel,
    2. Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel,
    3. Department of Clinical Biochemistry, Ben-Gurion University of the Negev, Beersheva, Israel
    Search for more papers by this author

Galila Agam PhD Psychiatry Research Unit, Beersheva Mental Health Center, PO Box 4600, Beersheva, Israel. Fax: +972 7 6400737; e-mail: galila@bgumail.bgu.ac.il

Abstract

Objectives: The study aimed to determine whether low inositol content and uptake previously reported in brain and peripheral tissue of bipolar patients are also reflected in lymphocyte-derived cell lines from these patients.

Methods:Inositol content and uptake were studied in lymphocyte-derived cell lines grown in vitro for at least five generations to eliminate influences of drug treatment. Inositol content was studied gas chromatographically and inositol uptake by following 3H-inositol incorporation at various concentrations.

Results: Inositol levels of cell lines derived from bipolar patients were significantly lower than those of cell lines from controls.

Conclusions: Low inositol content in lymphocyte-derived cell lines from bipolar patients corroborates previous findings in frontal cortex and in lymphoblastoid cell lines and are consistent with the notion that the phosphatidylinositol signaling system is involved in the pathophysiology of this disorder.

Ancillary