Get access

3′(2′)-Phosphoadenosine 5′-phosphate phosphatase is reduced in postmortem frontal cortex of bipolar patients

Authors

  • G Shaltiel,

    1. Stanley Research Center and Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel
    Search for more papers by this author
  • N Kozlovsky,

    1. Stanley Research Center and Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel
    Search for more papers by this author
  • R H Belmaker,

    1. Stanley Research Center and Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel
    Search for more papers by this author
  • G Agam

    1. Stanley Research Center and Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheva, Israel
    Search for more papers by this author

Galila Agam PhD Psychiatry Research Unit, Mental Health Center, PO Box 4600, Beersheva 84170, Israel. e-mail: galila@bgumail.bgu.ac.il

Abstract

Shaltiel G, Kozlovsky N, Belmaker RH, Agam G. 3′(2′)-Phosphoadenosine 5′-phosphate phosphatase is reduced in postmortem frontal cortex of bipolar patients. Bipolar Disord 2002: 4: 302–306. © Blackwell Munksgaard, 2002

Objective: 3′(2′)-Phosphoadenosine 5′-phosphate (PAP) phosphatase is a novel lithium (Li) inhibitable enzyme. Thus the enzyme seemed an important candidate for studies of the molecular etiology of bipolar disorder.

Methods: RT–PCR, Western-blot analysis and Pi liberation were used to measure PAP phosphatase mRNA levels, protein levels and enzyme activity (respectively) in postmortem frontal cortex specimens of bipolar patients versus normal subjects.

Results: The PAP phosphatase protein levels were 24% significantly lower in bipolar patients than in normal subjects. PAP phosphatase mRNA levels and enzymatic activity did not differ between normal controls and bipolar patients.

Conclusions: Abnormality of PAP phosphatase in bipolar patients offers a new direction for study of bipolar disorder etiology.

Ancillary