Typing of human papillomavirus in Zimbabwean patients with invasive cancer of the uterine cervix
Article first published online: 9 JUL 2003
Acta Obstetricia et Gynecologica Scandinavica
Volume 82, Issue 8, pages 762–766, August 2003
How to Cite
Stanczuk, G. A., Kay, P., Sibanda, E., Allan, B., Chirara, M., Tswana, S. A., Bergstrom, S. and Williamson, A.-L. (2003), Typing of human papillomavirus in Zimbabwean patients with invasive cancer of the uterine cervix. Acta Obstetricia et Gynecologica Scandinavica, 82: 762–766. doi: 10.1034/j.1600-0412.2003.00245.x
- Issue published online: 9 JUL 2003
- Article first published online: 9 JUL 2003
- Submitted 30 January, 2003Accepted 13 February, 2003
- cervical cancer;
Background. Cervical cancer affects 1 in 2000 Zimbabwean women. We investigated the type-specific distribution of human papillomavirus (HPV) infection in Zimbabwean women with invasive cervical cancer.
Methods. We conducted a descriptive study on 98 women with invasive cervical cancer. The methods used were a nested polymerase chain reaction (PCR) for amplification of HPV-DNA and restriction fragment length polymorphism (RFLP) to characterize the HPV types.
Results. HPV-DNA was identified in 97% of the cases. HPV types 16, 33, 18 and 31 were identified in 61%, 39%, 18% and 4% of the patients, respectively. We typed one case each of HPV types 35 and 58. Multiple HPV infections were present in 24%. All patients (n = 3) with adenocarcinoma of the cervix were infected with the HPV. Patients infected with HPV-16 alone presented at a median age of 46 years while those infected with HPV-33 alone presented at 43 years. However, patients coinfected with both HPV-16 and HPV-33 were between 10 and 13 years older (median age of 56 years) than patients with either HPV-16 or HPV-33 as single infections. These differences were marginally significant (p = 0.08) or significant (p = 0.02), respectively.
Conclusion. We present the first prevalence data on HPV types in patients with cervical cancer in Zimbabwe and show that, provided appropriate techniques are employed, HPV infection can be identified in a majority of the patients. The distribution of HPV types should be taken into consideration in tailoring locally relevant vaccines against HPV.