Characterization of vasomotion in porcine retinal arterioles
Article first published online: 29 MAY 2003
Acta Ophthalmologica Scandinavica
Volume 81, Issue 3, pages 278–282, June 2003
How to Cite
Hessellund, A., Jeppesen, P., Aalkjaer, C. and Bek, T. (2003), Characterization of vasomotion in porcine retinal arterioles. Acta Ophthalmologica Scandinavica, 81: 278–282. doi: 10.1034/j.1600-0420.2003.00063.x
- Issue published online: 29 MAY 2003
- Article first published online: 29 MAY 2003
- Received on August 22nd, 2002. Accepted on February 4th, 2003.
- retinal blood flow;
- porcine arterioles;
- in vitro experiments
Purpose: To characterize vasomotion in porcine retinal arterioles in vitro using isobaric (pressure myograph) and isometric (wire myograph) methods.
Methods: Pressure myograph: 208 small porcine retinal arterioles (outer diameter 68 ± 4 μm) were studied under isobaric conditions in a double-barrelled pipette system. Diameter changes of the arterioles were registered by video recordings. Wire myograph: 60 large porcine retinal arterioles (inner diameter 147 ± 1.6 μm) were studied under isometric conditions in a small vessel myograph for force measurements.
Results: The rates of success in initiating vasomotion were 7.2% using the pressure myograph and 43% using the wire myograph (p < 0.001). The small vessels studied under isobaric conditions oscillated with a frequency of 0.014 Hz and the episodes lasted 6.0 ± 1.0 min, whereas the large vessels under isometric conditions oscillated with a significantly faster frequency of 0.043 Hz and lasted 32.1 ± 4.9 min (p = 0.026).
Conclusion: Retinal vasomotion can be studied in vitro using both pressure myograph and wire myograph techniques. The wire myograph is superior to the pressure myograph in initiating and maintaining vasomotion in vitro.