Soluble membrane-type 1 matrix metalloproteinase (MT1-MMP) and gelatinase A (MMP-2) in induced sputum and bronchoalveolar lavage fluid of human bronchial asthma and bronchiectasis

Authors

  • PÄIVI MAISI,

    1. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki,
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  • KAIU PRIKK,

    1. Department of Oral and Maxillofacial Diseases, Biomedicum and Oral Pathology Unit, Laboratory Diagnostics, Helsinki University Central Hospital (HUCH), University of Helsinki, Orton Research Institute and the Orthopaedic Hospital of Invalid Foundation, Helsinki, Finland,
    2. Institute of Experimental and Clinical Medicine, Tallinn, Estonia,
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  • RUTH SEPPER,

    1. Institute of Experimental and Clinical Medicine, Tallinn, Estonia,
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  • EMMA PIRILÄ,

    1. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki,
    2. Department of Oral and Maxillofacial Diseases, Biomedicum and Oral Pathology Unit, Laboratory Diagnostics, Helsinki University Central Hospital (HUCH), University of Helsinki, Orton Research Institute and the Orthopaedic Hospital of Invalid Foundation, Helsinki, Finland,
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  • TUULA SALO,

    1. Departments of Oral Diagnostics and Medicine, and Pathology, University of Oulu, Oulu, Finland
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  • JARKKO HIETANEN,

    1. Department of Oral and Maxillofacial Diseases, Biomedicum and Oral Pathology Unit, Laboratory Diagnostics, Helsinki University Central Hospital (HUCH), University of Helsinki, Orton Research Institute and the Orthopaedic Hospital of Invalid Foundation, Helsinki, Finland,
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  • TIMO SORSA

    1. Department of Oral and Maxillofacial Diseases, Biomedicum and Oral Pathology Unit, Laboratory Diagnostics, Helsinki University Central Hospital (HUCH), University of Helsinki, Orton Research Institute and the Orthopaedic Hospital of Invalid Foundation, Helsinki, Finland,
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  • Received May 16, 2002.

    Accepted September 2, 2002.

* Timo Sorsa, Biomedicum and Institute of Dentistry, University of Helsinki, PO Box 63 (Haartmaninkatu 8), 00014 Helsinki University, Finland. e-mail: tsorsa@hammas.helsinki.fi

Abstract

The aim of this study was to investigate the involvement of the MT1-MMP/MMP-2 cascade in induced sputum (IS) and bronchoalveolar lavage fluid (BALF) from bronchial asthma (BA) and bronchiectasis (BE) patients and healthy controls. The molecular forms and cellular origins of MT1-MMP and MMP-2 were determined by Western immunoblotting, immunohistochemistry and in situ hybridization. Elevated levels of soluble activated and autocatalyzed MT1-MMP species as well as activated forms of MMP-2 in IS and BALF samples from BA and BE patients were evidenced. The activation degrees of soluble MT1-MMP and MMP-2 were significantly correlated in BA and BE IS and BALF. Only low levels of both these MMPs were observed in healthy control IS and BALF. The co-expression of MMP-2 with MT1-MMP was evidenced by double immunostaining in bronchial epithelial cells, submucosal glandular cells, smooth muscle cells and monocyte/macrophages. The MT1-MMP/MMP-2 cascade is present and active in human inflammatory lung disease fluid and tissue samples. This cascade seemingly reflects the active destructive phases of these chronic lung diseases.

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