Received December 30, 2002.Accepted April 10, 2003.
Determination of proliferation index in neoplasms using different Ki-67 equivalent antibodies
Article first published online: 25 JUL 2003
Volume 111, Issue 5, pages 567–570, May 2003
How to Cite
LINDBOE, C. F., VON DER OHE, G. and TORP, S. H. (2003), Determination of proliferation index in neoplasms using different Ki-67 equivalent antibodies. APMIS, 111: 567–570. doi: 10.1034/j.1600-0463.2003.1110505.x
- Issue published online: 25 JUL 2003
- Article first published online: 25 JUL 2003
- proliferation index;
- Ki-67; MIB-1
Paraffin sections from 23 tumours were immunohistochemically stained with the following four Ki-67 equivalent antibodies: monoclonal MIB-1 (DAKO), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (DAKO). Ki-67 labelling indices were determined by counting in exactly the same area in each case. MIB-1 showed the highest labelling index in 21 of the 23 cases, and the mean MIB-1 index was approximately 30% higher than that of the other antibodies. The differences between MM1, NCL-Ki-67p and Rah Ki-67 were small and non-significant. There was a positive correlation between each of the four antibodies. As these findings may be of importance when the Ki-67 labelling index is used as a criterion for tumour grading or for clinical prognostication, this necessitate identification of the antibody used in every case.