Simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles
Version of Record online: 20 AUG 2002
Volume 11, Issue 4, pages 381–385, August 2002
How to Cite
Magerl, M., Kauser, S., Paus, R. and Tobin, D. J. (2002), Simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles. Experimental Dermatology, 11: 381–385. doi: 10.1034/j.1600-0625.2002.110414.x
- Issue online: 20 AUG 2002
- Version of Record online: 20 AUG 2002
- Accepted for publication 9 October 2001
- dermal papilla;
- hair follicle papilla;
- hair follicle;
- cell culture;
Abstract: Study of the involvement of the hair follicle papilla in hair growth regulation was greatly facilitated by the isolation and cultivation of this tiny cluster of fibroblast-like cells in the rat vibrissae and in the human hair follicle. While isolation of the hair follicle papilla from the former is relatively straightforward, the current method to isolate the much smaller human hair follicle requires significant skill. Thus, the routine initiation of primary cultures of human scalp hair follicle papilla cells requires significant training, time, and commitment.
In an attempt to simplify hair follicle papilla cell culture methodology for new laboratory personnel, we have made significant refinements to the current method. Our method requires only two simple manipulations to isolate hair follicle papilla from intact isolated hair follicles. This very rapid and easy method isolates clean and intact hair follicle papillae. Together with their attachment via scratching to the growth surface, the isolation and cultivation of this important hair follicle component can now be achieved easily by the laboratory newcomer. The method relies for its simplicity on the removal of the hair follicle papilla from the outside of the intact hair follicle rather than via internal manipulations from within the hair follicle.