Expression of β Chemokines in Explants and Trophoblasts from Early and Term Human Placentae

Authors

  • MARLÉNE MOUSSA,

  • BARBARA MOGNETTI,

  • SYLVIE DUBANCHET,

  • ELISABETH MENU,

  • GÉRARD CHAOUAT,

  • ELISABETH MENU,

  • FRANÇOISE BARRE-SINOUSSI,

  • BARBARA MOGNETTI,

  • PIERRE ROQUES,

  • DOMINIQUE DORMONT


Address reprint requests to Marléne Moussa, INSERM U131, Equipe Cytokines et Relation Materno Fœtale, Maternité, Hôpital Antoine Béclère, Avenue de la Porte de Trivaux, 92140 Clamart, France

E-mail: marlene.moussa@inserm.ipsc.u-psud.fr

Abstract

PROBLEM: Implantation of human embryo requires expression of inflammatory cytokines and local attraction of T cells and natural killer (NK) cells. Chemokines are chemoattractants for these cells in classical inflammation. We speculated that they could also be involved in implantation.
METHOD OF STUDY: We assessed by enzyme-linked immunosorbent assay (ELISA), reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry the presence of three classical β chemokines Macrophage Inflammatory Protein 1 (MIP1)α, MIP1β and Regulated upon activation, normal T cells expressed and secreted (RANTES) in cultures of placental villi or isolated trophoblasts derived from human first trimester and term placenta.
RESULTS: Explant culture assays were positive for these three chemokines, with important quantitative variations between individuals. Half of the highly purified trophoblasts cultures were found by ELISA and RT-PCR to secrete in vitro MIP1α and MIP1β. RANTES was never detected by ELISA in trophoblasts cultures, albeit we could detect a low amount of messenger RNA. Immunohistochemistry experiments show that Hofbauer cells and the trophoblast layer are a secretion site of MIP1β in term placenta, and that cytotrophoblasts are able to secrete this chemokine in early placenta.
CONCLUSION: Human placenta is a site of secretion of chemokines that could be involved in establishment of pregnancy.

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