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IL-1 Activity is Expressed Differently During Pregnancy in the Rat Uterine Artery than in Aortic or Uterine Tissues

Authors

  • Mahmoud Huleihel,

    1. MAHMOUD HULEIHEL
      Department of Microbiology and Immunology, Soroka University Medical Center, Soroka, Israel,
    2. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Soroka, Israel,
    3. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel
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  • Joseph R. Leiberman,

    1. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Soroka, Israel,
    2. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel
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  • David Yohay,

    1. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Soroka, Israel,
    2. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel
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  • Gershon Holcberg,

    1. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Soroka, Israel,
    2. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel
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  • Miriam Katz,

    1. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Soroka, Israel,
    2. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel
    Search for more papers by this author
  • Moshe Mazor

    1. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Soroka, Israel,
    2. MAHMOUD HULEIHEL
      JOSEPH R. LEIBERMAN
      DAVID YOHAY
      GERSHON HOLCBERG
      MIRIAM KATZ
      MOSHE MAZOR
      Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel
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Address reprint requests to Mahmoud Huleihel, Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel. E-mail: huleihel@bgumail.bgu.ac.il

Abstract

PROBLEM: Uterine artery was shown to be unique in its capacity to change in size and function during pregnancy. As interleukin-1 (IL-1) was shown to be involved in reproduction processes, the aim of this study was to determine the levels of IL-1 activity of the uterine artery tissue in pregnant rat.

METHOD OF STUDY: Nine virgins and nine midpregnant rats were selected. Both uterine arteries were obtained, together with reference tissues from aorta and uterus. The levels of IL-1 were examined in the above tissues after culturing with media alone (control; CT), and media that contained stimulants like tumor necrosis factor-alpha (TNF-α) or lipopolysaccharide (LPS). IL-1-like activity was evaluated by its capacity to promote the culture growth of 1A-5 and cytotoxic T lymphocyte derived (CTLD) cell lines. This activity was expressed as optical density (OD)/mg protein of the examined organ.

RESULTS: Uterine artery tissue, of pregnant rats, cultured in medium alone produced significantly higher levels of IL-1 than uterine artery of virgin animals under the same conditions (16.2 S.E. 1.3 versus 0.6 S.E. 0.05 OD/mg protein, respectively; P < 0.02). Stimulation of uterine artery in vitro by LPS and TNF increased their capacity to secrete IL-1. In comparison with uterine artery, aorta produced higher levels of IL-1 in virgin rats compared with pregnant rats (13.6 S.E. 1.2 versus 1.6 S.E. 0.1; P < 0.02). Stimulation of aorta tissues (from both virgin and pregnant rats) with LPS, in vitro, significantly decreased their capacity to secrete IL-1 (P < 0.04). Stimulation of aorta tissues from virgin rats with TNF-α, in vitro, did not change their capacity to secrete IL-1 activity. However, stimulation of aorta tissues from pregnant rats with TNF-α decreased the secretion of bioactive IL-1. The levels of IL-1 produced by uterine tissues from virgin and pregnant rats were similar, and stimulation with either LPS or TNF-α significantly decreased their capacity to secrete IL-1 (P < 0.04).

CONCLUSIONS: The high level of IL-1 activity detected during pregnancy in the uterine artery may suggest its unique involvement in the changes occurring throughout pregnancy in those blood vessels.

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