Deficiency of Decidual IL-10 in First Trimester Missed Abortion: A Lack of Correlation with the Decidual Immune Cell Profile

Authors

  • MICHAEL PLEVYAK,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
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  • NAZEEH HANNA,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
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  • SANDRA MAYER,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • SHAUN MURPHY,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • HALIT PINAR,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • LOREN FAST,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • CHRISTINA EKERFELT,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • JAN ERNERUDH,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • GORAN BERG,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • LEIF MATTHIESEN,

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author
  • SURENDRA SHARMA

    1. MICHAEL PLEVYAK
      Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      NAZEEH HANNA*
      SANDRA MAYER
      SHAUN MURPHY
      HALIT PINAR
      SURENDRA SHARMA
      Department of Pediatrics, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA, HALIT PINAR
      SURENDRA SHARMA
      Department of Pathology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA,

      LOREN FAST
      Department of Medicine, Rhode Island Hospital, Brown University, Providence, RI, USA,

      CHRISTINA EKERFELT
      JAN ERNERUDH
      GORDAN BERG
      LEIF MATTHIESEN
      Divisions of Clinical Immunology and Gynecology and Obstetrics, Faculty of Health Sciences, University Hospital, Linkoping, Sweden
    Search for more papers by this author

Address reprint requests to Surendra Sharma, Department of Pediatrics, Women and Infants' Hospital-Brown University, 101 Dudley Street, Providence, RI 02905, USA. E-mail: ssharma@wihri.org

Abstract

PROBLEM: To determine if first trimester missed abortion decidua is characterized by an altered immune cell profile and/or a modified interleukin (IL)-10 and interferon (IFN)-γ production pattern compared with decidua from elective termination.

METHOD OF STUDY: Flow cytometry and immunohistochemistry techniques were used to determine the decidual immune cell phenotypic profile and production pattern of IL-10 and IFN-γ in cases of elective termination (n=14) and missed abortion (n=12).

RESULTS: Both groups had a similar proportion of CD56+ CD16, CD56+ CD16+, CD19+, CD3+, CD4+, CD8+, αβ T cells and γδ T cells. The majority of αβ and γδ positive T cells in both groups coexpressed the natural killer (NK) cell marker CD56, but lacked cell surface expression of CD3. Diminished decidual IL-10 staining was noted in 7/10 missed abortion cases compared with none of the elective termination cases (n=12) (P=0.007). A uniform decidual IFN-γ staining pattern was observed in both groups.

CONCLUSION: Decreased IL-10 production coupled with a sustained IFN-γ presence noted in missed abortion compared with elective termination cases suggest that these cytokines may be important determinants in pregnancy outcome. In contrast, differences in the proportion of immune cells between both groups may not be a critical factor in early pregnancy loss. In normal pregnancy, decidual αβ and γδ positive T cells with reduced CD3 on their cell surface may be intrinsically restricted in T-cell receptor (TCR)-mediated activation.

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