Physiological Leukocytosis during Pregnancy is Associated with Changes in Glucose Transporter Expression of Maternal Peripheral Blood Granulocytes and Monocytes

Authors

  • EMIN T. KORGUN,

    1. EMIN T. KORGUN, PETER SEDLMAYR, GOTTFRIED DOHR, TOM HAHN, Institute of Histology and Embryology, University of Graz, Austria,
    2. EMIN T. KORGUN, RAMAZAN DEMIR, Institute of Histology and Embryology, Akdeniz University, Antalya, Turkey,
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  • RAMAZAN DEMIR,

    1. EMIN T. KORGUN, RAMAZAN DEMIR, Institute of Histology and Embryology, Akdeniz University, Antalya, Turkey,
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  • PETER SEDLMAYR,

    1. EMIN T. KORGUN, PETER SEDLMAYR, GOTTFRIED DOHR, TOM HAHN, Institute of Histology and Embryology, University of Graz, Austria,
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  • GERNOT DESOYE,

    1. GERNOT DESOYE, GUERKAN ARIKAN, PETER PUERSTNER, MARTIN HAEUSLER, Department of Obstetrics and Gynecology, University of Graz, Graz, Austria,
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  • GUERKAN ARIKAN,

    1. GERNOT DESOYE, GUERKAN ARIKAN, PETER PUERSTNER, MARTIN HAEUSLER, Department of Obstetrics and Gynecology, University of Graz, Graz, Austria,
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  • PETER PUERSTNER,

    1. GERNOT DESOYE, GUERKAN ARIKAN, PETER PUERSTNER, MARTIN HAEUSLER, Department of Obstetrics and Gynecology, University of Graz, Graz, Austria,
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  • MARTIN HAEUSLER,

    1. GERNOT DESOYE, GUERKAN ARIKAN, PETER PUERSTNER, MARTIN HAEUSLER, Department of Obstetrics and Gynecology, University of Graz, Graz, Austria,
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  • GOTTFRIED DOHR,

    1. EMIN T. KORGUN, PETER SEDLMAYR, GOTTFRIED DOHR, TOM HAHN, Institute of Histology and Embryology, University of Graz, Austria,
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  • GERHARD SKOFITSCH,

    1. GERHARD SKOFITSCH, Institute of Zoology, University of Graz, Graz, Austria
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  • TOM HAHN

    1. EMIN T. KORGUN, PETER SEDLMAYR, GOTTFRIED DOHR, TOM HAHN, Institute of Histology and Embryology, University of Graz, Austria,
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Tom Hahn Institute of Histology and Embryology, University of Graz, Harrachgasse 21, A-8010 Graz, Austria. E-mail: tom.hahn@kfunigraz.ac.at

Abstract

PROBLEM: The scarce data on glucose transporter expression of leukocytes are contradictory and nothing is known about changes accompanying physiological leukocytosis during pregnancy, which imposes acute metabolic demands on the cells.

METHOD OF STUDY: Cytospin preparations of intravascular leukocytes were searched immunocytochemically for the high affinity glucose transporters GLUT1, 3 and 4. Pregnancy-associated quantitative changes in transporter expression were assessed by flow cytometry.

RESULTS: Granulocytes and monocytes stained for GLUT1, 3 and 4. Major changes in cell surface transporter expression during pregnancy were a 36% (P < 0.05) down-regulation of granulocyte GLUT1 at term, and an increase in monocyte GLUT3 levels to 137% (P < 0.05), paralleled by a 24% (P < 0.05) decrease in GLUT4 content in second trimester. Apart from a minor subpopulation, lymphocytes were negative for these carriers.

CONCLUSION: GLUT1, 3 and 4 are abundantly expressed in granulocytes and monocytes. The particular isoforms are differentially regulated during pregnancy, suggesting an individual functional significance.

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