Effects of Cytokines on VEGF Expression and Secretion by Human First Trimester Trophoblast Cell Line
Version of Record online: 4 JUL 2002
American Journal of Reproductive Immunology
Volume 48, Issue 2, pages 70–76, August 2002
How to Cite
CHOI, S. J., PARK, J. Y., LEE, Y. K., CHOI, H. I., LEE, Y. S., KOH, C.-M. and CHUNG, I.-B. (2002), Effects of Cytokines on VEGF Expression and Secretion by Human First Trimester Trophoblast Cell Line. American Journal of Reproductive Immunology, 48: 70–76. doi: 10.1034/j.1600-0897.2002.01071.x
- Issue online: 4 JUL 2002
- Version of Record online: 4 JUL 2002
- vascular endothelial growth factor
PROBLEM: The mechanism through which vascular endothelial growth factor (VEGF) regulation occurs at the feto-maternal interface is poorly understood. The aim of this study was to investigate the effects of various cytokines on VEGF expression and secretion by trophoblast cells.
METHOD OF STUDY: We investigated the effects of cytokines on VEGF expression in human first trimester trophoblast cell line by analyzing VEGF messenger RNA (mRNA) by reverse transcription-polymerase chain reaction and VEGF protein secretion by enzyme linked immunosorbent assay.
RESULTS: The trophoblast cells expressed VEGF mRNA constitutively and the main subtypes were identified as VEGF121 and VEGF165. When cultured in the presence of interferon (IFN)-γ, interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-2, or IL-10, VEGF mRNA expression was found to be significantly increased by IL-1β, IFN-γ and TNF-α but to be unaffected by IL-2 and IL-10. Moreover, VEGF secretion was most significantly increased by IFN-γ treatment.
CONCLUSION: These results suggest that IL-1β, IFN-γ, and TNF-α may regulate the production of VEGF in early gestational trophoblasts.