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Increased Expression of Cyclooxygenase-2 in Local Lesions of Endometriosis Patients

Authors

  • FUMIHISA CHISHIMA,

    1. FUMIHISA CHISHIMA, SATOSHI HAYAKAWA, KENJI SUGITA, TATSUO YAMAMOTO, Department of Obstetrics and Gynecology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
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  • SATOSHI HAYAKAWA,

    1. FUMIHISA CHISHIMA, SATOSHI HAYAKAWA, KENJI SUGITA, TATSUO YAMAMOTO, Department of Obstetrics and Gynecology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
    2. SATOSHI HAYAKAWA, MITSUO HONDA, AIDS Research Center, National Institute of Infectious Disease, Koyama Shinjuku-ku, Tokyo, Japan
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  • KENJI SUGITA,

    1. FUMIHISA CHISHIMA, SATOSHI HAYAKAWA, KENJI SUGITA, TATSUO YAMAMOTO, Department of Obstetrics and Gynecology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
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  • NORIKO KINUKAWA,

    1. NORIKO KINUKAWA, SHEIKE ALEEMUZZAMAN, NORIMICHI NEMOTO, Department of Pathology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
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  • SHEIKE ALEEMUZZAMAN,

    1. NORIKO KINUKAWA, SHEIKE ALEEMUZZAMAN, NORIMICHI NEMOTO, Department of Pathology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
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  • NORIMICHI NEMOTO,

    1. NORIKO KINUKAWA, SHEIKE ALEEMUZZAMAN, NORIMICHI NEMOTO, Department of Pathology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
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  • TATSUO YAMAMOTO,

    1. FUMIHISA CHISHIMA, SATOSHI HAYAKAWA, KENJI SUGITA, TATSUO YAMAMOTO, Department of Obstetrics and Gynecology, Nihon University School of Medicine, Oyaguchi-kamimachi, Itabashi-ku, Tokyo, Japan,
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  • MITSUO HONDA

    1. SATOSHI HAYAKAWA, MITSUO HONDA, AIDS Research Center, National Institute of Infectious Disease, Koyama Shinjuku-ku, Tokyo, Japan
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Fumihisa Chishima Department of Obstetrics and Gynecology, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. E-mail: fchishi@med.nihon-u.ac.jp

Abstract

PROBLEM: Human endometrial glands contain the highest levels of cyclooxygenase (COX), although whether it is COX-1 and/or COX-2 has not been previously determined. Overexpression of COX-2 may result in the pathogenesis of endometriosis.

METHOD OF STUDY: Tissue sections were obtained from 28 premenopausal women undergoing laparotomy or laparoscopic surgery for benign conditions. Endometrium, ectopic endometriosis tissue and peritoneum were obtained at the time of surgery. Informed consents were obtained from all the patients participating in this study. Immunohistochemistry was performed on consecutive sections of paraffin-embedded tissue using anti-COX-2 antibody. Expressions of COX-2 mRNA in endometrium, ectopic endometriosis tissue, and peritoneum were quanti-tavely determined by competitive reverse transcription–polymerase chain reaction (RT–PCR).

RESULTS: In the uterus, COX-2 was localized in the endometrial epithelium. Eutopic endometrial surface epithelium contains more COX-2 than does glandular epithelium. We observed more frequent and denser COX-2 staining in the ectopic endometriosis implants when compared with eutopic endometrium. Level of COX-2 mRNA in endometriosis was increased up to five times that of eutopic endometria.

CONCLUSION: Hyper activation of COX-2 with abnormal prostaglandin generation is considered to contribute to the pathophysiology of endometriosis and disease progression.

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