Get access

Effect of an Endocrine Disruptor on Mammalian Fertility. Application of Monoclonal Antibodies against Sperm Proteins as Markers for Testing Sperm Damage

Authors

  • JANA PEKNICOVÁ,

    1. JANA PEKNICOVÁ
      VENDULA KYSELOVÁ
      MICHAEL BOUBELÍK
      Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic,
    Search for more papers by this author
  • VENDULA KYSELOVÁ,

    1. JANA PEKNICOVÁ
      VENDULA KYSELOVÁ
      MICHAEL BOUBELÍK
      Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic,
    Search for more papers by this author
  • DANA BUCKIOVÁ,

    1. DANA BUCKIOVÁ
      Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Search for more papers by this author
  • MICHAEL BOUBELÍK

    1. JANA PEKNICOVÁ
      VENDULA KYSELOVÁ
      MICHAEL BOUBELÍK
      Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic,
    Search for more papers by this author

Address reprint requests to Jana Peknicová, Department of Biology and Biochemistry of Fertilization, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeeská 1083, 142 20 Prague 4, Czech Republic. E-mail: jpeknic@biomed.cas.cz

Abstract

PROBLEM:  To determine the influence of an endocrine disruptor [bisphenol-A (BPA)] on the integrated reproductive process as well as on individual reproductive organs and gametes in order to select suitable markers for testing sperm damage.

METHOD OF STUDY:  The effect of BPA on fertility in vivo in multigenerational studies in an outbred stock of mice was studied. Damage of reproductive organs was assessed by histochemical methods and damage of spermatozoa by means of a panel of monoclonal antibodies (MoAbs) against intra-acrosomal sperm proteins.

RESULTS:  BPA had a negative influence on offspring born of mice, on reproductive organs, and on acrosome integrity of mice spermatozoa. Selected MoAbs against intra-acrosomal mammalian sperm proteins, cross-reacted with mouse spermatozoa, were used for determination of the acrosome integrity. BPA had no effect on body weight and testicle weight of males.

CONCLUSIONS:  The present results demonstrate that BPA has a negative effect on in vivo fertility of mice, with impact on spermatogenesis and sperm quality. Monoclonal antibodies against intra-acrosomal sperm proteins can be used for detecting sperm damage.

Ancillary