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Absence of HLA-G Expression in Macrophages of Human Decidua

Authors

  • PETER SEDLMAYR,

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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  • PEDRO MORALES,

    1. PEDRO MORALES, Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, USA,
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  • SILVIA TRUMMER,

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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  • KARIN WASCHER,

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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  • DAGMAR AZZOLA,

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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  • ASTRID BLASCHITZ,

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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  • ASTRID HAMMER,

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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  • WOLFGANG WALCHER,

    1. WOLFGANG WALCHER, Department of Obstetrics and Gynecology, Karl-Franzens-University, Graz, Austria
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  • REINHOLD WINTERSTEIGER,

    1. REINHOLD WINTERSTEIGER, Institute of Pharmaceutical Chemistry, Karl-Franzens-University, Graz, Austria,
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  • GOTTFRIED DOHR

    1. PETER SEDLMAYR, SILVIA TRUMMER, KARIN WASCHER, DAGMAR AZZOLA, ASTRID BLASCHITZ, ASTRID HAMMER, GOTTFRIED DOHR, Institute of Histology and Embryology, Karl-Franzens-University, Graz, Austria,
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Dr Peter Sedlmayr, Institute of Histology and Embryology, Karl-Franzens University, Harrachgasse 21, A-8010 Graz, Austria. E-mail: peter.sedlmayr@kfunigraz.ac.at

Abstract

PROBLEM: Macrophages – together with natural killer (NK) cells – constitute the majority of bone marrow derived infiltrating cells in the decidua. As interferon-γ (IFN-γ), a cytokine produced by NK cells, has been reported to induce expression of human leukocyte antigen (HLA-G) in monocytic cells, suggesting expression of HLA-G on decidua macrophages potentially stimulated by IFN-γ, the question arises whether decidua macrophages in normal pregnancy express HLA-G.

METHOD OF STUDY: The study was based on immunohistochemistry and flow cytometry. In order to exclude that potentially elusive soluble HLA-G was not detected by immunohistochemistry, we performed in addition RT–PCR of flow-sorted decidua macrophages.

RESULTS: Our findings indicate that HLA-G is not present on macrophages of first trimester or term decidua in either membrane-bound or soluble form. Transcripts for soluble HLA-G1 and -G2 were not detected.

CONCLUSIONS: We exclude a role of HLA-G on the surface of decidua macrophages or of soluble HLA-G1 or -G2 as a secretory product of decidua macrophages with regard to interaction with HLA-G receptors present in or outside the decidua.

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