Dominant IL-10 and TGF-β mRNA Expression in γδT Cells of Human Early Pregnancy Decidua Suggests Immunoregulatory Potential
Article first published online: 19 JUN 2002
American Journal of Reproductive Immunology
Volume 48, Issue 1, pages 9–17, July 2002
How to Cite
NAGAEVA, O., JONSSON, L. and MINCHEVA-NILSSON, L. (2002), Dominant IL-10 and TGF-β mRNA Expression in γδT Cells of Human Early Pregnancy Decidua Suggests Immunoregulatory Potential. American Journal of Reproductive Immunology, 48: 9–17. doi: 10.1034/j.1600-0897.2002.01131.x
- Issue published online: 19 JUN 2002
- Article first published online: 19 JUN 2002
- Submitted October 10, 2001, revised October 31, 2001, accepted November 2, 2001
- γδT cells;
- human pregnancy;
PROBLEM: To examine the cytokine gene expression in γδT-cells from human early pregnancy decidua.
METHOD OF STUDY: The cytokine messenger RNA (mRNA) expression in isolated decidual T-cell receptor (TCR)γδ+/CD56+ and TCRγδ single positive cells was investigated with a panel of cytokine primers and probes selected to distinguish between T helper (Th)1, Th2, Th3 and regulatory T-cells (Tr1) type of immune response using real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS: TCRγδ+/CD56+ cells express almost exclusively the immunosuppressive cytokines interleukin-10 (IL-10) and transforming growth factor (TGF)-β. The TCRγδ single positive cells enhance their transcription of IL-10 and TGF-β, compared with the TCRγδ+/CD56+ cells and additionally express mRNA for IL-1β and IL-6.
CONCLUSIONS: The present findings suggest that γδT cells in normal pregnancy create a cytokine milieu promoting immunotolerance to the fetus. We hypothesize that through the production of the immunosuppressive cytokines IL-10 and/or TGF-β the γδT cells could function directly as regulatory T cells or induce the differentiation of Th0 TCRαβ+ cells into regulatory/suppresser cells.