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Induction of Transferrin Secretion in Murine Sertoli Cells by FSH and IL-1: The Possibility of Different Mechanism(s) of Regulation

Authors

  • MAHMOUD HULEIHEL,

    1. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      Department of Microbiology and Immunology, Soroka University Medical Center, Beer-Sheva, Israel,
    2. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MELANIE ZEYSE
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva, Israel,
    3. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MOSHE MAZOR
      Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel,
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  • DANIEL ZEYSE,

    1. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      Department of Microbiology and Immunology, Soroka University Medical Center, Beer-Sheva, Israel,
    2. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MELANIE ZEYSE
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva, Israel,
    3. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MOSHE MAZOR
      Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel,
    4. DANIEL ZEYSE
      MELANIE ZEYSE
      Christian-Albrechts-University of Kiel Medical School, Kiel, Germany
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  • EITAN LUNENFELD,

    1. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MELANIE ZEYSE
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva, Israel,
    2. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MOSHE MAZOR
      Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel,
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  • MELANIE ZEYSE,

    1. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MELANIE ZEYSE
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva, Israel,
    2. DANIEL ZEYSE
      MELANIE ZEYSE
      Christian-Albrechts-University of Kiel Medical School, Kiel, Germany
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  • MOSHE MAZOR

    1. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MELANIE ZEYSE
      MOSHE MAZOR
      Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva, Israel,
    2. MAHMOUD HULEIHEL
      DANIEL ZEYSE
      EITAN LUNENFELD
      MOSHE MAZOR
      Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel,
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Address reprint requests to: M. Huleihel, Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. E-mail: huleihel@bgumail.bgu.ac.il

Abstract

In the present study we examined the capacity of interleukin-1 (IL-1) α, β, interleukin-1 receptor antagonist (IL-1ra) and follicle stimulating hormone (FSH) to induce transferrin secretion by Sertoli cells under in vitro conditions. Primary Sertoli cell (SC) cultures from immature mice secreted constitutively transferrin. Stimulation of these cultures with IL-1α, IL-1β significantly increas\d their capacity to secrete transferrin. Addition of IL-1ra to unstimulated SC cultures did not affect their capacity to secrete transferrin. Stimulation of SC cultures with a combination of both IL-1α and FSH or IL-1β and FSH showed additive effect between IL-1 and FSH in their capacity to induce transferrin secretion by these cells. However, stimulation of Sertoli cells with a combination of both IL-1ra and FSH did not affect their capacity to secrete transferrin compared with FSH-stimulated cultures.

Our results may suggest the involvement of testicular paracrine/autocrine factors (IL-1) and endocrine (FSH) factors in the regulation of transferrin secretion by SC. This capacity seems to be differently regulated by these factors.

Thus, IL-1α and β may directly affect physiological functions of the testis; which may suggest their involvement in the regulation of spermatogenesis and spermiogenesis processes and male fertility.

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