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Changes in the Number of Polymorphonuclear Leukocytes and Concentrations of IL-8 and Granulocyte Elastase in the Vaginas of Normal Pregnant Women

Authors

  • TETSUO YAMADA,

    1. TETSUO YAMADA
      HISANORI MINAKAMI
      SHIGEKI MATSUBARA
      YASUHIRO KOHMURA
      TOSHIO AOYA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
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  • HISANORI MINAKAMI,

    1. TETSUO YAMADA
      HISANORI MINAKAMI
      SHIGEKI MATSUBARA
      YASUHIRO KOHMURA
      TOSHIO AOYA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
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  • SHIGEKI MATSUBARA,

    1. TETSUO YAMADA
      HISANORI MINAKAMI
      SHIGEKI MATSUBARA
      YASUHIRO KOHMURA
      TOSHIO AOYA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
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  • YASUHIRO KOHMURA,

    1. TETSUO YAMADA
      HISANORI MINAKAMI
      SHIGEKI MATSUBARA
      YASUHIRO KOHMURA
      TOSHIO AOYA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
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  • TOSHIO AOYA,

    1. TETSUO YAMADA
      HISANORI MINAKAMI
      SHIGEKI MATSUBARA
      YASUHIRO KOHMURA
      TOSHIO AOYA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
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  • IKUO SATO

    1. TETSUO YAMADA
      HISANORI MINAKAMI
      SHIGEKI MATSUBARA
      YASUHIRO KOHMURA
      TOSHIO AOYA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
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Address reprint request to: Tetsuo Yamada, Department of Obstetrics and Gynecology, Jichi Medical School, Minamikawachimachi, Tochigi, 329-0498, Japan. E-mail: yamada88@jichi.ac.jp

Abstract

PROBLEM: It is unknown whether the number of vaginal polymorphonuclear leukocytes (vPMNs) of healthy pregnant women changes during pregnancy.

METHOD OF STUDY: We studied 23 women after 14.7 ± 3.6 weeks, 37 women after 25.2 ± 3.1 weeks, and 26 women after 32.9 ± 2.0 weeks of gestation. Numbers of total vPMNs, viable vPMNs, dead vPMNs, percentage viability of vPMNs, and concentrations of IL-8 and elastase activity were determined in vaginal washings.

RESULTS: The vPMNs, including viable and dead vPMNs, significantly increased with advancing gestation from 9.9 ± 8.6 × 105 to 13.1 ± 13.4 × 105, and to 27.1 ± 26.6 × 105 (r=0.315, P < 0.01). Vaginal IL-8 significantly increased with advancing gestation. However, increases in vaginal elastase activity did not occur.

CONCLUSIONS: These results suggested that an increase in vPMNs is an early event, and that the increase in vaginal elastase activity is a late event in relation to the ripening of the uterine cervix.

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