Interleukin-12 Secretion by Peripheral Blood Mononuclear Cells is Decreased in Normal Pregnant Subjects and Increased in Preeclamptic Patients

Authors

  • MASATOSHI SAKAI,

    1. MASATOSHI SAKAI
      HIROSHI TSUDA
      KYOKO TANEBE
      YASUSHI SASAKI
      SHIGERU SAITO
      Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Sugitani Toyama-shi, Toyama, Japan
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  • HIROSHI TSUDA,

    1. MASATOSHI SAKAI
      HIROSHI TSUDA
      KYOKO TANEBE
      YASUSHI SASAKI
      SHIGERU SAITO
      Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Sugitani Toyama-shi, Toyama, Japan
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  • KYOKO TANEBE,

    1. MASATOSHI SAKAI
      HIROSHI TSUDA
      KYOKO TANEBE
      YASUSHI SASAKI
      SHIGERU SAITO
      Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Sugitani Toyama-shi, Toyama, Japan
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  • YASUSHI SASAKI,

    1. MASATOSHI SAKAI
      HIROSHI TSUDA
      KYOKO TANEBE
      YASUSHI SASAKI
      SHIGERU SAITO
      Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Sugitani Toyama-shi, Toyama, Japan
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  • SHIGERU SAITO

    1. MASATOSHI SAKAI
      HIROSHI TSUDA
      KYOKO TANEBE
      YASUSHI SASAKI
      SHIGERU SAITO
      Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Sugitani Toyama-shi, Toyama, Japan
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Address reprint requests to: Shigeru Saito, Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, 2630 Sugitani Toyama-shi, Toyama 930-0194, Japan. E-mail: s30saito@ms.toyama-mpu.ac.jp

Abstract

PROBLEM:  It has been reported that T-helper (Th) 2 dominance in normal pregnancy shifts to Th1 dominance in preeclampsia. Peripheral blood mononuclear cell (PBMC) production of interleukin (IL)-12, which induce Th1 responses, has not been compared between these clinical states.

METHOD OF STUDY:  Peripheral blood mononuclear cell from 35 non-pregnant women, 35 healthy pregnant women, 12 mildly preeclamptic patients, and 15 severely preeclamptic patients were cultured for 24 hr. IL-12 secretion was determined by enzyme-linked immunosorbent assay (ELISA). Th1/Th2 ratios in PBMC were determined flow-cytometrically, and the amounts of HLA-DR and CD14 expression on the monocytes were obtained by flow cytometry.

RESULTS:  Peripheral blood mononuclear cell from healthy pregnant subjects secreted less IL-12 than non-pregnant women. PBMC from severely preeclamptic patients secreted more IL-12 than those from healthy pregnant subjects, while IL-12 secretion in mild preeclampsia resembled secretion in normal pregnancy. Th1/Th2 ratios correlated were positively with IL-12. Increased HLA-DR antigens and reduced CD14 expression, suggesting monocyte activation, were observed in preeclamptic patients, although monocyte counts were unchanged.

CONCLUSION:  Decreased IL-12 secretion by PBMC may cause Th2 dominance in normal pregnancy, while increased IL-12 secretion by activated monocytes may cause Th1 dominance in preeclampsia.

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