Genetic Contribution of the Interleukin-10 Promoter Polymorphism in Endometriosis Susceptibility

Authors

  • JO KITAWAKI,

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • HIROSHI OBAYASHI,

    1. HIROSHI OBAYASHI
      GOJI HASEGAWA
      NAOTO NAKAMURA
      TOSHIKAZU YOSHIKAWA
      The First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan,
    2. HIROSHI OBAYASHI
      Department of Clinical Research, Kyoto Microbiological Institute, Kyoto, Japan,
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  • MITSUHIRO OHTA,

    1. MITSUHIRO OHTA
      Department of Clinical Chemistry, Kobe Pharmaceutical University, Kobe, Japan
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  • NORIKO KADO,

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • HIROAKI ISHIHARA,

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • HISATO KOSHIBA,

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • IZUMI KUSUKI,

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • KATSUMI TSUKAMOTO,

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • GOJI HASEGAWA,

    1. HIROSHI OBAYASHI
      GOJI HASEGAWA
      NAOTO NAKAMURA
      TOSHIKAZU YOSHIKAWA
      The First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • NAOTO NAKAMURA,

    1. HIROSHI OBAYASHI
      GOJI HASEGAWA
      NAOTO NAKAMURA
      TOSHIKAZU YOSHIKAWA
      The First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • TOSHIKAZU YOSHIKAWA,

    1. HIROSHI OBAYASHI
      GOJI HASEGAWA
      NAOTO NAKAMURA
      TOSHIKAZU YOSHIKAWA
      The First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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  • HIDEO HONJO

    1. JO KITAWAKI
      NORIKO KADO
      HIROAKI ISHIHARA
      HISATO KOSHIBA
      IZUMI KUSUKI
      KATSUMI TSUKAMOTO
      HIDEO HONJO
      Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
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Address reprint requests to Jo Kitawaki, Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan. E-mail: kitawaki@koto.kpu-m.ac.jp

Abstract

PROBLEM: Interleukin-10 (IL-10) is an important immunomodulatory cytokine. The aim of this study was to investigate whether polymorphisms of the IL-10 gene promoter polymorphisms may be responsible in part for genetic susceptibility to endometriosis.

METHODS OF STUDY: Polymorphisms at position −1082 and −592 in the IL-10 promoter region were determined in 196 patients with endometriosis and 160 fertile healthy women by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).

RESULTS: There were no statistically significant differences in the genotype and allele frequencies of IL-10 promoter polymorphism between the endometriosis and control groups. However, when subgrouped according to clinical features, the frequencies of the −592*CC genotype and −592*C allele were significantly increased in patients with autoantibodies to carbonic anhydrase II (anti-CA II ab) compared with controls.

CONCLUSION: IL-10 promoter polymorphisms were associated with the production of anti-CA II ab in patients with endometriosis, suggesting a role in the genetic susceptibility for endometriosis.

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