Diversity of Antisperm Antibodies Bound to Sperm Surface in Male Immunological Infertility

Authors

  • HIROAKI SHIBAHARA,

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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  • TETSUO TSUNODA,

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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  • AKIYO TANEICHI,

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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  • YUKI HIRANO,

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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  • AKIKO OHNO,

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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  • SATORU TAKAMIZAWA,

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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  • CHIEKO YAMAGUCHI,

    1. CHIEKO YAMAGUCHI
      HIROMICHI TSUNODA
      Department of Clinical Laboratory, Jichi Medical School, Tochigi, Japan
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  • HIROMICHI TSUNODA,

    1. CHIEKO YAMAGUCHI
      HIROMICHI TSUNODA
      Department of Clinical Laboratory, Jichi Medical School, Tochigi, Japan
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  • IKUO SATO

    1. HIROAKI SHIBAHARA
      TETSUO TSUNODA
      AKIYO TANEICHI
      YUKI HIRANO
      AKIKO OHNO
      SATORU TAKAMIZAWA
      IKUO SATO
      Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan,
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Address reprint requests to Hiroaki Shibahara, Department of Obstetrics and Gynecology, Jichi Medical School, 3311-1, Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan.E-mail: sibahara@jichi.ac.jp

Abstract

PROBLEM: The presence of antisperm antibodies (ASA) in males can reduce fecundity, however, relationship between the two is disputed. This study was performed to investigate if there is diversity of ASA bound to sperm surface using immunobead test (IBT) combined with complement dependent sperm immobilization test (SIT).

METHODS: The ASA bound to sperm surface were detected using the direct IBT (D-IBT) in 275 semen samples. In some cases with ASA detected by D-IBT, sperm immobilizing antibodies bound to sperm surface were also evaluated using direct SIT (D-SIT).

RESULTS: The incidence of the immunoglobulin G (IgG), IgA, and IgM classes of ASA detected by D-IBT were 2.5, 1.8, and 0.4%, respectively. Totally, nine (3.3%) infertile men had ASA on the sperm surface. D-SIT was tested positive in four (66.7%) of six cases with ASA assessed by D-IBT.

CONCLUSIONS: Some of the sperm-bound antibodies are associated with complement dependent sperm immobilizing antibodies, indicating that there exists a heterogeneity of sperm-bound antibodies. This result might be one of the reasons for the controversy about the relationship between ASA and immunological infertility in men.

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