Macrophages in Human Reproductive Tissues Contain Luteinizing Hormone/Chorionic Gonadotropin Receptors

Authors

  • Y. M. Zhang,

    1. Division of Research, Department of Obstetrics, Gynecology and Women's Health, University of Louisville Health Sciences Center, Louisville, KY, USA
    Search for more papers by this author
  • Ch. V. Rao,

    1. Division of Research, Department of Obstetrics, Gynecology and Women's Health, University of Louisville Health Sciences Center, Louisville, KY, USA
    Search for more papers by this author
  • Z. M. Lei

    1. Division of Research, Department of Obstetrics, Gynecology and Women's Health, University of Louisville Health Sciences Center, Louisville, KY, USA
    Search for more papers by this author

Address reprint requests to Ch.V. Rao, Division of Research, Department of Ob/Gyn and Women's Health, 438 MDR Building, 511 South Floyd Street, University of Louisville Health Sciences Center, Louisville, KY 40292, USA.
E-mail: cvrao001@gwise.louisville.edu
Present address: Y.M. Zhang, Department of Ob/Gyn, St Michael's Hospital, University of Toronto, Toronto, Canada.

Abstract

PROBLEM: To determine whether macrophages in human reproductive tissues contain luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptor mRNA and receptor protein that can bind 125I-hCG.

METHOD OF STUDY: Macrophages isolated from term pregnancy human decidua were used for LH/hCG receptor detection by in situ hybridization for receptor mRNA and immunocytochemistry for a macrophage marker, CD68, performed alone and in combination, reverse transcription-nested polymerase chain reaction, Western and ligand blotting. The LH/hCG receptor presence in macrophages in late luteal phase human endometria and corpora lutea was determined by sequential performance of in situ hybridization and immunocytochemistry.

RESULTS: The macrophages present in term pregnancy human decidua and late luteal phase human endometria and corpora lutea contain LH/hCG receptors.

CONCLUSIONS: This is the first demonstration of macrophages present in human reproductive tissues containing LH/hCG receptors. The receptor presence suggests that LH and hCG may regulate macrophage functions in gonadal as well as in non-gonadal target tissues.

Ancillary