Macrophages in Human Reproductive Tissues Contain Luteinizing Hormone/Chorionic Gonadotropin Receptors
Article first published online: 21 FEB 2003
American Journal of Reproductive Immunology
Volume 49, Issue 2, pages 93–100, February 2003
How to Cite
Zhang, Y. M., Rao, Ch. V. and Lei, Z. M. (2003), Macrophages in Human Reproductive Tissues Contain Luteinizing Hormone/Chorionic Gonadotropin Receptors. American Journal of Reproductive Immunology, 49: 93–100. doi: 10.1034/j.1600-0897.2003.00013.x
- Issue published online: 21 FEB 2003
- Article first published online: 21 FEB 2003
- Submitted February 25, 2002; revised April 19, 2002; accepted June 3, 2002.
- Corpus luteum;
- human macrophages;
- luteinizing hormone/ human chorionic gonadotropin receptors
PROBLEM: To determine whether macrophages in human reproductive tissues contain luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptor mRNA and receptor protein that can bind 125I-hCG.
METHOD OF STUDY: Macrophages isolated from term pregnancy human decidua were used for LH/hCG receptor detection by in situ hybridization for receptor mRNA and immunocytochemistry for a macrophage marker, CD68, performed alone and in combination, reverse transcription-nested polymerase chain reaction, Western and ligand blotting. The LH/hCG receptor presence in macrophages in late luteal phase human endometria and corpora lutea was determined by sequential performance of in situ hybridization and immunocytochemistry.
RESULTS: The macrophages present in term pregnancy human decidua and late luteal phase human endometria and corpora lutea contain LH/hCG receptors.
CONCLUSIONS: This is the first demonstration of macrophages present in human reproductive tissues containing LH/hCG receptors. The receptor presence suggests that LH and hCG may regulate macrophage functions in gonadal as well as in non-gonadal target tissues.