Intracellular Expression of CD69 in Endometrial and Peripheral T cells Represents a Useful Marker in Women with Recurrent Miscarriage: Modulation After Allogeneic Leukocyte Immunotherapy
Article first published online: 17 MAR 2003
American Journal of Reproductive Immunology
Volume 49, Issue 3, pages 149–158, March 2003
How to Cite
Ramhorst, R., García, V., Agriello, E., Corigliano, A., Etchepareborda, E., Irigoyen, M., Pasanante, G. and Fainboim, L. (2003), Intracellular Expression of CD69 in Endometrial and Peripheral T cells Represents a Useful Marker in Women with Recurrent Miscarriage: Modulation After Allogeneic Leukocyte Immunotherapy. American Journal of Reproductive Immunology, 49: 149–158. doi: 10.1034/j.1600-0897.2003.00021.x
- Issue published online: 17 MAR 2003
- Article first published online: 17 MAR 2003
- Submitted May 03, 2002; revised July 22, 2002; accepted July 22, 2002
- maternal allogeneic response;
- recurrent spontaneous abortions
PROBLEM: To characterize in fertile women and women with recurrent spontaneous abortions (RSA) the expression and functional status of T cells expressing the CD69 molecule.
METHOD OF STUDY: We analyzed by flow cytometry in peripheral blood and endometrium from fertile and RSA women, the surface and cytoplasmic expression of CD69 on gated T cells. In addition, we investigated by three-color flow cytometry the expression of cytokines, and subsets of memory T cells.
RESULTS: In T cells, CD69 was restricted to the intracellular compartment with a higher frequency in RSA than in fertile women (68.2 ± 12% versus 23.7 ± 22%, P < 0.001, and 20 ± 9.5% versus 2.1 ± 3.8%, P < 0.005, in endometrium and peripheral blood, respectively). In contrast, the number of interferon-γ+ (IFN-γ+) secreting cells was higher (16 ± 5% versus 6 ± 1%) in fertile women. All 11 RSA women alloimmunized with parental leukocytes reached values of CD3+ CD69+ cells similar to those observed in fertile women.
CONCLUSIONS: CD69 might represent a useful marker in the diagnosis and the follow up of RSA patients.