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Intracellular Expression of CD69 in Endometrial and Peripheral T cells Represents a Useful Marker in Women with Recurrent Miscarriage: Modulation After Allogeneic Leukocyte Immunotherapy

Authors

  • R. Ramhorst,

    1. Division of Immunogenetics, Hospital de Clínicas ‘José de San Martín’, University of Buenos Aires,
    2. Department of Microbiology, School of Medicine, University of Buenos Aires,
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  • V. García,

    1. Division of Immunogenetics, Hospital de Clínicas ‘José de San Martín’, University of Buenos Aires,
    2. Department of Microbiology, School of Medicine, University of Buenos Aires,
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  • E. Agriello,

    1. Department of Microbiology, School of Medicine, University of Buenos Aires,
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  • A. Corigliano,

    1. Department of Microbiology, School of Medicine, University of Buenos Aires,
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  • E. Etchepareborda,

    1. Division of Gynecology, Hospital de Clínicas ‘José de San Martín’, University of Buenos Aires, Buenos Aires, Argentina
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  • M. Irigoyen,

    1. Division of Gynecology, Hospital de Clínicas ‘José de San Martín’, University of Buenos Aires, Buenos Aires, Argentina
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  • Gómez Pasanante,

    1. Division of Gynecology, Hospital de Clínicas ‘José de San Martín’, University of Buenos Aires, Buenos Aires, Argentina
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  • L. Fainboim

    1. Division of Immunogenetics, Hospital de Clínicas ‘José de San Martín’, University of Buenos Aires,
    2. Department of Microbiology, School of Medicine, University of Buenos Aires,
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Address reprint requests to Leonardo Fainboim, División Inmunogenética, Hospital de Clínicas, Avenue Córdoba 2351, piso 3° (1120) Buenos Aires, Argentina. E-mail: inmuno@fmed.uba.ar

Abstract

PROBLEM:  To characterize in fertile women and women with recurrent spontaneous abortions (RSA) the expression and functional status of T cells expressing the CD69 molecule.

METHOD OF STUDY:  We analyzed by flow cytometry in peripheral blood and endometrium from fertile and RSA women, the surface and cytoplasmic expression of CD69 on gated T cells. In addition, we investigated by three-color flow cytometry the expression of cytokines, and subsets of memory T cells.

RESULTS:  In T cells, CD69 was restricted to the intracellular compartment with a higher frequency in RSA than in fertile women (68.2 ± 12% versus 23.7 ± 22%, P < 0.001, and 20 ± 9.5% versus 2.1 ± 3.8%, P < 0.005, in endometrium and peripheral blood, respectively). In contrast, the number of interferon-γ+ (IFN-γ+) secreting cells was higher (16 ± 5% versus 6 ± 1%) in fertile women. All 11 RSA women alloimmunized with parental leukocytes reached values of CD3+ CD69+ cells similar to those observed in fertile women.

CONCLUSIONS:  CD69 might represent a useful marker in the diagnosis and the follow up of RSA patients.

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