Granulocyte colony stimulation factor (G-CSF) suppresses interleukin (IL)-12 and/or IL-2 induced interferon (IFN)-γproduction and cytotoxicity of decidual mononuclear cells
Article first published online: 16 JUN 2003
American Journal of Reproductive Immunology
Volume 50, Issue 1, pages 83–89, July 2003
How to Cite
Sugita, K., Hayakawa, S., Karasaki-Suzuki, M., Hagiwara, H., Chishima, F., Aleemuzaman, S., Li, J.-a., Nishinarita, S. and Yamamoto, T. (2003), Granulocyte colony stimulation factor (G-CSF) suppresses interleukin (IL)-12 and/or IL-2 induced interferon (IFN)-γproduction and cytotoxicity of decidual mononuclear cells. American Journal of Reproductive Immunology, 50: 83–89. doi: 10.1034/j.1600-0897.2003.00024.x
- Issue published online: 16 JUN 2003
- Article first published online: 16 JUN 2003
- Submitted 20 May, 2002; revised 15 July, 2002; accepted 28 August, 2002.
- Granulocyte colony stimulating factor;
- decidual lymphocytes;
Problem: The placenta is one of the few non-hematopoietic tissues to express granulocyte colony stimulation factor (G-CSF). Placental G-CSF production is considered to be one of the major causes of granulocytosis during pregnancy although its physiological role in pregnancy has not yet been examined.
Method of study: The effects of G-CSF on interleukin (IL)-2 and/or IL-12 induced interferon (IFN)-γ production of magnetic cell sorting (MACS) sorted decidual lymphocytes was examined by enzyme-linked immunosorbent spot-forming cell assay (ELISPOT). The effect of G-CSF on cytotoxicity of decidual lymphocytes against the choriocarcinoma cell line JEG-3 was examined by lactate dehydrogenase (LDH) release assay.
Results: As previously reported by us, IL-2 and/or IL-12 activated decidual mononuclear cells were capable of killing choriocarcinoma cells. We observed that G-CSF abolished IFN-γ production and cytotoxicity of decidual mononuclear cells and MACS sorted CD56+ cells.
Conclusions: In addition to its well-known trophic effects on hematopoiesis, our results suggest about new roles of G-CSF in reproductive immunology.