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In vitro Regulation of β1 and β3 Integrin Subunits in Endometrial Epithelial Cells From Normal Endometrium


Address reprint requests to Antonio Lanzone, Istituto di Ginecologia ed Ostetricia, Università Cattolica S. Cuore, L.go A. Gemelli, 8, Roma 00168 Italy.


OBJECTIVE:  To evaluate the effect of prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) on integrin expression.

DESIGN:  Cultures of endometrial epithelial cells from normal endometrium.

SETTING:  All endometrial specimens were obtained from the Obstetrics and Gynecology Department of the Catholic University, Rome, Italy.

PATIENTS:  Four patients with normal menstrual cycles undergoing operative laparoscopy for non-endometrial problems.

INTERVENTION:  Endometrial samples were collected by uterine courettings.

MAIN OUTCOME MEASURE:  Immunocytochemistry for β1 and β3 integrin subunits.

RESULTS:  PGE2 clearly enhances both β1 and β3 integrin subunit expression. IL-1β seem to slightly increase only β3 subunit expression.

CONCLUSIONS:  In light of the critical role played by eicosanoids in endometrial differentiation, we suggest that PGE2 is also involved in local paracrine regulation of integrin expression.

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