OBJECTIVE: To evaluate the effect of prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) on integrin expression.
DESIGN: Cultures of endometrial epithelial cells from normal endometrium.
SETTING: All endometrial specimens were obtained from the Obstetrics and Gynecology Department of the Catholic University, Rome, Italy.
PATIENTS: Four patients with normal menstrual cycles undergoing operative laparoscopy for non-endometrial problems.
INTERVENTION: Endometrial samples were collected by uterine courettings.
MAIN OUTCOME MEASURE: Immunocytochemistry for β1 and β3 integrin subunits.
RESULTS: PGE2 clearly enhances both β1 and β3 integrin subunit expression. IL-1β seem to slightly increase only β3 subunit expression.
CONCLUSIONS: In light of the critical role played by eicosanoids in endometrial differentiation, we suggest that PGE2 is also involved in local paracrine regulation of integrin expression.