Increased T-Cell Activation in Decidua Parietalis Compared to Decidua Basalis in Uncomplicated Human Term Pregnancy
Article first published online: 9 APR 2003
American Journal of Reproductive Immunology
Volume 49, Issue 5, pages 261–268, May 2003
How to Cite
Sindram-Trujillo, A., Scherjon, S., Kanhai, H., Roelen, D. and Claas, F. (2003), Increased T-Cell Activation in Decidua Parietalis Compared to Decidua Basalis in Uncomplicated Human Term Pregnancy. American Journal of Reproductive Immunology, 49: 261–268. doi: 10.1034/j.1600-0897.2003.00041.x
- Issue published online: 9 APR 2003
- Article first published online: 9 APR 2003
- Submitted June 25, 2002;revised October 8, 2002;accepted November 11, 2002.
- Decidua basalis;
- decidua parietalis;
- immunology of pregnancy;
- T-cell activation
PROBLEM: The aim of this study was to quantify and compare activated T cells in term decidua basalis and parietalis using flow cytometry.
METHOD OF STUDY: Term decidua basalis and parietalis samples were obtained from 20 placentas collected after elective caesarean section. Percentages of leukocyte subclasses within the CD45+ cell fraction and activated T cells were determined by flow cytometry.
RESULTS: There was no significant difference in CD45+, CD14+, CD19+, and CD3+ cell percentages. However, within the CD3+ population, there were significantly more T-cell receptor-γδ+ (TCR-γδ+) and CD8+ cells in decidua parietalis compared with decidua basalis. More importantly, percentages of T cells expressing CD25, human leukocyte antigen (HLA)-DR, CD45RO, and CD69 markers were significantly increased in decidua parietalis.
CONCLUSION: These findings suggest that there are more activated T cells in decidua parietalis than in decidua basalis. Further investigation into differences between the two decidual sites may expand our understanding of the immunology of the maternal–fetal interface.