Stimulatory Effect of the Herbal Medicine Keishi-bukuryo-gan on a Cytokine-induced Neutrophil Chemoattractant, in Rat Ovarian Cell Culture
Article first published online: 16 JUN 2003
American Journal of Reproductive Immunology
Volume 50, Issue 1, pages 90–97, July 2003
How to Cite
Yasui, T., Matsuzaki, T., Ushigoe, K., Kuwahara, A., Maegawa, M., Furumoto, H., Aono, T. and Irahara, M. (2003), Stimulatory Effect of the Herbal Medicine Keishi-bukuryo-gan on a Cytokine-induced Neutrophil Chemoattractant, in Rat Ovarian Cell Culture. American Journal of Reproductive Immunology, 50: 90–97. doi: 10.1034/j.1600-0897.2003.00055.x
- Issue published online: 16 JUN 2003
- Article first published online: 16 JUN 2003
- Submitted July 22, 2002; revised December 2, 2002; accepted December 6, 2002.
- Cytokine-induced neutrophil chemoattractant;
- tumor necrosis factor α
Problem and Method of Study: We investigated the effects of Keishi-bukuryo-gan, a Japanese herbal medicine, and its crude ingredients in relation to the production of cytokine-induced neutrophil chemoattractant (CINC/gro), interleukin 1β (IL-1β) and tumor necrosis factor α (TNFα), which are known to stimulate the secretion of CINC/gro in the ovulatory process, and the effects of Keishi-bukuryo-gan with those of Toki-shakuyaku-san, which has been shown to have an effect on the ovary. We cultured whole ovarian dispersates from immature (3-week-old) female rats with Keishi-bukuryo-gan, Toki-shakuyaku-san and crude ingredients of Keishi-bukuryo-gan. The contents of CINC/gro, IL-1β and TNFα in the cultured media were determined by enzyme-linked immunosorbent assay (ELISA).
Results: Keishi-bukuryo-gan stimulated the secretion of CINC/gro in a dose-dependent manner, and the secretion of CINC/gro into the culture medium increased significantly at concentrations of Keishi-bukuryo-gan of 10 and 100 μg/mL (P < 0.001). The stimulatory effect of Keishi-bukuryo-gan on the production of CINC/gro is significantly (P < 0.001) stronger than that of Toki-shakuyaku-san at the same concentrations of 100 μg/mL. In addition, Keishi-bukuryo-gan stimulated the secretion of IL-1β in a dose-dependent manner, while it did not stimulate the secretion of TNFα even at a concentration of 100 μg/mL. Moutan Cortex, Paeoniae Radix and Persicae Semen, which are crude ingredients of Keishi-bukuryo-gan, enhanced the secretion of CINC/gro significantly (P < 0.01) in cultured whole ovarian dispersates.
Conclusions: These results show that Keishi-bukuryo-gan can stimulate the secretion of CINC/gro as well as the production of IL-1β and that this stimulatory effect of Keishi-bukuryo-gan was significantly stronger than that of Toki-shakuyaku-san in immature rat ovarian cell culture.