Immunization of Naïve Mice with Mouse Laminin-1 Affected Pregnancy Outcome in a Mouse Model
Version of Record online: 7 JUL 2003
American Journal of Reproductive Immunology
Volume 50, Issue 2, pages 159–165, August 2003
How to Cite
Matalon, S. T., Blank, M., Matsuura, E., Inagaki, J., Nomizu, M., Levi, Y., Koike, T., Shere, Y., Ornoy, A. and Shoenfeld, Y. (2003), Immunization of Naïve Mice with Mouse Laminin-1 Affected Pregnancy Outcome in a Mouse Model. American Journal of Reproductive Immunology, 50: 159–165. doi: 10.1034/j.1600-0897.2003.00068.x
- Issue online: 7 JUL 2003
- Version of Record online: 7 JUL 2003
- Revised February 10, 2003; accepted February 19, 2003.
- Animal model;
- anti-laminin antibodies;
- fetal loss;
PROBLEM: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure.
METHOD OF STUDY: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome.
RESULTS: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P < 0.01). Laminin-1 immunized mice also had lower fetal and placental weights.
CONCLUSIONS: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.